Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2021 Feb;82(2):282-327.
doi: 10.1016/j.jinf.2020.09.004. Epub 2020 Sep 10.

A possible role for GRP78 in cross vaccination against COVID-19

Abdo A Elfiky  1 , Ibrahim M Ibrahim  2 , Alaa M Ismail  2 , Wael M Elshemey  3
Affiliations
Comment

A possible role for GRP78 in cross vaccination against COVID-19

Abdo A Elfiky et al. J Infect. 2021 Feb.
No abstract available

PubMed Disclaimer

Conflict of interest statement

Declaration of Competing Interest The authors declare that there is no competing interest in this work.

Figures

Fig. 1
Fig. 1
Coronavirus spike protein and GRP78 recognition site. (A) Part of the multiple sequence alignment for the spike glycoproteins of the seven reported human coronavirus strains (NL63, 229E, OC43, HKU1, MERS-CoV, SARS-CoV, and SARS-CoV-2). Yellow columns are the conserved residues among the seven HCoVs. GRP78 recognition site (C480-C488 in SARS-CoV-2) is marked at the top. (B) Pairwise sequence alignment between Pep42 and SARS-CoV-2 S (C480-C488 region). Red and yellow residues are identical and similar residues, respectively. Fingerprint residues are marked (bottom). (C) The structure of spike protein RBD (rose) bound to ACE2 (cyan) and B(0)AT1 (green) (PDB ID: 6M17) while the GRP78 recognition site (C480-C488 in spike protein RBD) is depicted in the blue sticks in the enlarged panel. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
Fig. 2
Fig. 2
The docked complex of GRP78 (red) and S RBD (magenta) superimposed to the solved structure (PDB ID: 6M17) containing spike protein RBD (rose), ACE2 (cyan), and B(0)AT1 (green). (A) shows the cartoon representation, while (B) indicates the surface representation. The C480-C488 are labeled with its one-letter code and shown in blue. The membrane is depicted in (B) to show how the CS-GRP78 would look like when binding the spike protein RBD. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
See this image and copyright information in PMC

Comment on

References

    1. Ibrahim I.M., Abdelmalek D.H., Elshahat M.E., Elfiky A.A. COVID-19 spike-host cell receptor GRP78 binding site prediction. J Infect. 2020;80(5):554–562. - PMC - PubMed
    1. Braun J., Loyal L., Frentsch M., Wendisch D., Georg P., Kurth F. SARS-CoV-2-reactive T cells in healthy donors and patients with COVID-19. Nature. 2020 - PubMed
    1. Elfiky A.A., Baghdady A.M., Ali S.A., Ahmed M.I. GRP78 targeting: hitting two birds with a stone. Life Sci. 2020 - PMC - PubMed
    1. Quinones Q.J., Ridder G.G.d., Pizzo S.V. GRP78, a chaperone with diverse roles beyond the endoplasmic reticulum. Histol Histopathol. 2008 - PubMed
    1. Kim Y., Lillo A.M., Steiniger S.C., Liu Y., Ballatore C., Anichini A. Targeting heat shock proteins on cancer cells: selection, characterization, and cell-penetrating properties of a peptidic GRP78 ligand. Biochemistry. 2006;45(31):9434–9444. - PubMed

Substances