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. 2020 Aug 9:27:e00513.
doi: 10.1016/j.btre.2020.e00513. eCollection 2020 Sep.

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Ismail B Onajobi  1 , Esther O Idowu  1 , Jamiu O Adeyemi  1 , Oyindamola J Samson  1 , Peter I Ogunyinka  2 , Obasola E Fagade  3
Affiliations

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

Ismail B Onajobi et al. Biotechnol Rep (Amst). .

Abstract

This study aims to screen bacterial isolates from Olabisi Onabanjo University Farmland for antibacterial activity against pathogenic microorganisms. Agar well diffusion method was used. Isolates were identified molecularly. Chi-square test revealed significant association between isolates, antibacterial activity with likelihood p-value = 0.000 and 5% significant level. Six among thirty-five isolates exhibited antibacterial activity against the test pathogenic species. A greater antibacterial activity (50 % inhibition) was observed in Lysinibacillus sphearicus strain PRE16. It inhibited the growth of Bacillus subtilis, Staphylococcus aureus and Escherichia coli by 23.00 ± 2.00, 18.00 ± 2.00 and 20.00 ± 4.00 respectively. DNA sequencing revealed antagonist isolates as Bacillus sp. BCN2, Brochothrix thermosphacta strain P30C4, Bacillus aryabhattai strain KNUC205, Alcaligenes faecalis strain KEM24, Bacillus arsenicus strain CSD05 and Lysinibacillus sphaericus strain PRE16. Phylogenetic analysis revealed close relatedness of most isolates with Bacillus species strains. These strains are suggested to be effective for the discovery of new antibacterial agents.

Keywords: Analysis; Antibacterial; Electrophoresis; Identification; Molecular; Pathogenic; Phylogenetic.

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Conflict of interest statement

The authors report no declarations of interest.

Figures

Fig. 1
Fig. 1
Map of sample location with the google map Uniform locator https://goo.gl/maps/qwbkbkMcvPZ37Hyk7.
Fig. 2
Fig. 2
Gel image of PCR purified DNA product. Key: M = Molecular marker 900bp molecular size; Is5, 1 s 8, Is 9, Is 10, Is 27 and Is 35= Isolate 5, 8, 9, 10, 27 and 35 respectively.
Plate 1
Plate 1
Antibacterial activity of some isolates against Bacillus subtilis.
Plate 2
Plate 2
Antibacterial activity of some isolates against Staphylococcus aureus.
Plate 3
Plate 3
Antibacterial activity of some isolates against Pseudomonas aeruginosa.
Fig. 3
Fig. 3
Full gel image of PCR purified DNA product. Key: Is5, 1 s8, Is9, Is10, Is27 and Is 35 = Isolate 5, 8, 9, 10, 27 and 35 respectively
Fig. 4
Fig. 4
Phylogenetic tree constructed using MEGA 5.05 based on homologous sequences of antibacterial potent isolates. Keys: B2= Isolate (Is) 5; D21= Isolate (Is) 35; D45= Isolate (Is) 8; D46= Isolate (Is) 9; H34= Isolate (Is) 27; D47= Isolate (Is) 10
See this image and copyright information in PMC

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