doi: 10.1073/pnas.74.6.2283.
Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction
- PMID: 329277
- PMCID: PMC432154
- DOI: 10.1073/pnas.74.6.2283
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Inactivation and proteolytic cleavage of phage lambda repressor in vitro in an ATP-dependent reaction
Proc Natl Acad Sci U S A.
1977 Jun.
Abstract
We have reproduced in vitro the inactivation of bacteriophage lambda repressor that occurs in vivo when a lambda lysogen is treated with agents such as ultraviolet radiation that attack DNA. ATP and a divalent cation are required for the inactivation reaction. The ind- repressor is insensitive to the inactivation mechanism. A proteolytic cleavage of repressor accompanies inactivation in vitro, as it does in vivo.
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