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Randomized Controlled Trial
. 2020 Sep 11;17(18):6619.
doi: 10.3390/ijerph17186619.

Evaluation of Salivary Matrix Metalloproteinase (MMP-8) in Periodontal Patients Undergoing Non-Surgical Periodontal Therapy and Mouthwash Based on Ozonated Olive Oil: A Randomized Clinical Trial

Gianna Maria Nardi  1 , Francesca Cesarano  1 , Giulio Papa  1 , Lorella Chiavistelli  1 , Roman Ardan  2 , Maciej Jedlinski  3 , Marta Mazur  1 , Roberta Grassi  4 , Felice Roberto Grassi  5
Affiliations
Randomized Controlled Trial

Evaluation of Salivary Matrix Metalloproteinase (MMP-8) in Periodontal Patients Undergoing Non-Surgical Periodontal Therapy and Mouthwash Based on Ozonated Olive Oil: A Randomized Clinical Trial

Gianna Maria Nardi et al. Int J Environ Res Public Health. .

Abstract

Background: Extracellular matrix metalloproteinases (MMPs) play a pivotal role in the damage to the periodontal tissue in patients with periodontitis. Scaling and root planning (SRP) attempt to control the plaque amount and consequentially reduce the bacterial load. Non-surgical periodontal treatment could be integrated with drug therapy and physiotherapy procedures such as ozone therapy. The aim of this study was to evaluate in a cohort of patients with a diagnosis of periodontitis: (1) the efficacy of non-surgical periodontal therapy assisted by the use of ozonated olive oil-based mouthwash on salivary metalloproteinase (MMP-8) and (2) the reduction of periodontal indices. Methods: Ninety-six subjects with a diagnosis of periodontitis were enrolled in this study and randomly assigned to the study group (SRP + mouthwash) or control group (SRP). The study duration was 3 months. Data on MMP-8, plaque index (PI), bleeding on probing (BoP) and probing pocket depth (PPD) were recorded at T0, T1 (14 days), T2 (1 month) and T3 (6 months). Group differences were assessed using Student's t-test for independent samples. Results: A significant improvement in PI, BoP, PPD and salivary MMP-8 levels was observed in both groups. An analysis of differences in relative changes of indices revealed the efficacy of ozonated olive oil in decreasing MMP-8 level. Simultaneously, it slowed the decrease of BoP index. Conclusions: Scaling and root plaining with the aid of ozonated olive oil mouthwash were found to be more effective on salivary MMP-8 reduction than scaling and root plaining alone.

Keywords: RCT; olive oil; ozonated oil; ozone; periodontitis; salivary metalloproteinase.

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Conflict of interest statement

The authors declare no conflict of interest related to this study.

Figures

Figure 1
Figure 1
Changes in the distribution of periodontal indices (a–c) and salivary metalloproteinase (MMP-8) levels (d).
Figure 1
Figure 1
Changes in the distribution of periodontal indices (a–c) and salivary metalloproteinase (MMP-8) levels (d).
See this image and copyright information in PMC

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