Catechol modified quaternized chitosan enhanced wet adhesive and antibacterial properties of injectable thermo-sensitive hydrogel for wound healing

Abstract

Wound dressings based on injectable thermo-sensitive hydrogel possess several advantages over preformed conventional dressings such as rapid reversible sol-gel transition behavior and the capacity of filling the irregular wound defect. Nevertheless, its clinical application is hindered by the weak tissue adhesiveness. Therefore, in this study, the catechol modified quaternized chitosan (QCS-C) was fabricated and incorporated into poly(d,l-lactide)-poly(ethylene glycol)-poly(d,l-lactide) (PLEL) hydrogel to develop an injectable hydrogel with the properties of thermo-sensitive, antibacterial and tissue adhesive. QCS-C could lower the LCST of hydrogel for easy gelation at physiological temperature, and significantly enhanced the tissue adhesion. For wound generation, nano-scaled bioactive glass (nBG：80 SiO₂, 16 CaO and 4 P₂O₅; mol%) was loaded into hydrogel to promote angiogenesis. The mice partial laceration experiment showed that PLEL-nBG-QCS-C hydrogel could effectively seal the ruptured skin and significantly accelerate wound healing. Thus, our findings established a new type of clinical treatment technology for complicated wounds.

Keywords: 3,4-Dihydroxyhydrocinnamic acid (CHA) (PubChem CID:348154); Adhesive; Calcium nitrate tetrahydrate (CN) (PubChem CID:16211656); Chitosan; Chitosan (PubChem CID:71853); Dodecylamine (DDA) (PubChem CID:13583); Glycidyltrimethylammonium chloride (GTMAC) (PubChem CID:18205); HCl (PubChem CID:313); Hydrogel; N-(3-dimethylaminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC) (PubChem CID:2723939); NaOH (PubChem CID:14798); Tetraethyl orthosilicate (TEOS) (PubChem CID:6517); Thermo-sensitive; Triethylphosphate (TEP) (PubChem CID:6535); Wound dressing.