Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Aug 26:157:27-41.
doi: 10.3897/phytokeys..55254. eCollection 2020.

Reassessment of Bournea Oliver (Gesneriaceae) based on molecular and palynological evidence

Wen-Hong Chen  1   2 , Ya-Mei Zhang  1   3 , Shi-Wei Guo  1   3 , Zhi-Rong Zhang  4 , Li Chen  1   5 , Yu-Min Shui  1   2
Affiliations

Reassessment of Bournea Oliver (Gesneriaceae) based on molecular and palynological evidence

Wen-Hong Chen et al. PhytoKeys. .

Abstract

The former genus Bournea is endemic to China, including two species, has been under consideration for incorporation into the expanded genus Oreocharis s.l. in Gesneriaceae. The phylogenetic tree inferred from two DNA sequences (trnL-F and ITS) showed that this genus is deeply nested into Oreocharis s.l. However, the new tree from seven ones (atpB-rbcL, ndhH-rps15-ycf1, rpl132, trnC-trnD, trnL-F, trnT-trnL of chloroplast DNA and ITS regions) revealed that Bournea is the sister group of other of Oreocharis s.l. Furthermore, Bournea is morphologically different from other Oreocharis based on existing data. We suggest keeping Bournea as an independent genus in Gesneriaceae.

Keywords: Oreocharis; Bournea, morphological characters of flowers; phylogeny; pollen grains.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
The morphology of Bournea sinensis Oliv. (A–E) and B. leiophylla (W. T. Wang) W. T. Wang (F–J). A plant B inflorescence C calyx and disc D stigma E front view of corolla showing the anthers and the style and stamens F plant G inflorescence H pistil and disc I stigma J front view of corolla showing the anthers.
Figure 2.
Figure 2.
The Bayes inference (BI) and Maximum likelihood (ML) tree inferred from six cp DNA markers (atpB-rbcL, ndhH-rps15-ycf1, rpl132, trnC-trnD, trnL-F, trnT-trnL) of the expanded genus Oreocharis s.l. in Gesneriaceae. Note 1) the red clade indicates the position of Bournea in phylogenetic trees; 2) the number of the node respectively indicates posterior probability values in BI and bootstrap values in ML, ※ indicates < 50%.
Figure 3.
Figure 3.
The Bayes inference (BI) and Maximum likelihood (ML) tree inferred from six cp DNA markers (atpB-rbcL, ndhH-rps15-ycf1, rpl132, trnC-trnD, trnL-F, trnT-trnL) and ITS of the expanded genus Oreocharis s.l. in Gesneriaceae. Note 1) the red clade indicates the position of Bournea in phylogenetic trees; 2) the number of the node respectively indicates posterior probability values in BI and bootstrap values in ML, ※ indicates < 50%.
Figure 4.
Figure 4.
The morphology of pollen grains of Bournea sinensis Oliv. (A–D) and B. leiophylla (W. T. Wang) W. T. Wang (E–H) by SEM. A polar view showing pollen grain with three equatorial, colporus apertures B equatorial view showing single free, prolate pollen grain C equatorial view show apertures and granular aperture membrane D detail showing verrucate tectum with granular E polar view showing pollen grain with three equatorial, colporus apertures F equatorial view showing single free, oblate pollen grain G equatorial view show apertures and granular aperture membrane H detail showing verrucate tectum with granular.
See this image and copyright information in PMC

References

    1. Bankevich A, Nurk S, Antipov D, Gurevich AA, Dvorkin M, Kulikov AS, Lesin VM, Nikolenko SI, Pham S, Prjibelski AD, Pyshkin AV, Sirotkin AV, Vyahhi N, Tesler G, Alekseyev MA, Pevzner PA. (2012) SPAdes: A new genome assembly algorithm and its applications to single-cell sequencing. Journal of Computational Biology 19(5): 455–477. 10.1089/cmb.2012.0021 - DOI - PMC - PubMed
    1. Chen WH, Shui YM, Sima YK, Zhang RM, Wei ZD. (2009) Pararuellia glomerata (Acanthaceae), a new species from Yunnan, China. Botanical Studies (Taipei, Taiwan) 50: 261–267.
    1. Chen WH, Shui YM, Yang JB, Wang H, Nishii K, Wen F, Zhang ZR, Möller M. (2014) Taxonomic status, phylogenetic affinities and genetic diversity of a presumed extinct genus, Paraisometrum W.T. Wang (Gesneriaceae) from the karst regions of Southwest China. PLoS One 9(9): E107967. 10.1371/journal.pone.0107967 - DOI - PMC - PubMed
    1. Doyle JJ, Doyle JL. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.
    1. Guo YF, Wang YQ. (2013) Supplemental study on pollen morphology of Epithandra (Gesneriaceae). Guihaia 33(4): 538–542.

LinkOut - more resources