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. 2021 Feb;259(2):443-448.
doi: 10.1007/s00417-020-04926-y. Epub 2020 Sep 16.

Conjunctival bacterial flora and their antibiotic sensitivity among patients scheduled for cataract surgery in a tertiary hospital in south-east Nigeria

Cyril Chukwukama Mamah  1 , Okey Charles Anyalebechi  1 , Stella Ngozi Onwubiko  2 , Mary Nkeiruka Okoloagu  1 , F C Maduka-Okafor  1 , S O Ebede  3 , R E Umeh  1
Affiliations

Conjunctival bacterial flora and their antibiotic sensitivity among patients scheduled for cataract surgery in a tertiary hospital in south-east Nigeria

Cyril Chukwukama Mamah et al. Graefes Arch Clin Exp Ophthalmol. 2021 Feb.

Abstract

Purpose: To determine the pre-operative conjunctival bacteria flora and their antibiotic susceptibility among patients scheduled for cataract surgery.

Methods: A descriptive, cross-sectional, hospital-based study design was adopted. It involved 104 consecutive, consented patients scheduled for cataract surgery at the University of Nigeria Teaching Hospital Enugu, from June to September 2017. The participants' socio-demographic characteristics, medical, and social history were obtained through interviewer-administered questionnaire. Conjunctival swab was obtained from the inferior fornix of the eye scheduled for cataract surgery using sterile swab stick and transported to medical laboratory for analysis. Susceptibility tests were carried out on the representatives of different groups of available antibiotics. Data was analyzed using the SPSS version 23.

Result: The participants were mainly females (64.4%), married, and farmers, with at least primary school education and a mean age of 64 ± 2SD years. The conjunctival swab bacteria isolation rate was 36.5%. Gram-positive bacteria accounted for 73.7% of the total isolates. Coagulase-negative Staphylococcus (34.2%) was the most frequently isolated bacteria. Gentamicin, ofloxacin, and polymycin B (76%) were the most efficacious antibiotics followed by moxifloxacin and tobramycin (68%) on all the isolates. Ofloxacin (82%) was the most sensitive to Gram positive while gentamicin (90%) was for Gram-negative bacteria.

Conclusion: In Enugu, the most common pre-operative conjunctival bacteria isolated were Coagulase-negative Staphylococcus with varied antibiotic sensitivity. Routine prophylaxis with gentamicin and ofloxacin is therefore advised to forestall the dreaded post-operative endophthalmitis.

Keywords: Cataract surgery; Conjunctival bacteria; Enugu Nigeria.

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References

    1. Mariotti S, Pascolini D (2012) Visual impairment, vision loss and blindness 2010 global estimates, and VI and blindness causes. Global data on visual impairments 2010. Br J Ophthalmol 96(5):614–618 - DOI
    1. Mahdi AM, Rabiu M, Gilbert C, Sivasubramaniam S, Murthy GV, Ezelum C, Entekume G (2014) Nigeria National Blindness and Visual Impairment Study Group. Prevalence and risk factors for lens opacities in Nigeria: results of the national blindness and low vision survey. Invest Ophthalmol Vis Sci 55(4):2642–2651 - DOI
    1. Speaker MG, Milch FA, Shah MK, Eisner W, Kreiswirth BN (1991) Role of external bacterial flora in the pathogenesis of acute postoperative endophthalmitis. Ophthalmology. 98:639–649 - DOI
    1. Results of the Endophthalmitis Vitrectomy Study (1995) A randomized trial of immediate vitrectomy and of intravenous antibiotics for the treatment of postoperative bacterial endophthalmitis. Endophthalmitis Vitrectomy Study Group. Arch Ophthalmol 113(12):1479–1496 - DOI
    1. Haggag M, Ali MI, Eweis M, Hassanein B (2011) Microflora isolated from preoperative conjunctivas, the prevalence of methicilin-resistant Staphylococci and their antibiotic profile. J Am Sci 7(12):579–584

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