Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2020 Jul 27;21(1):505-514.
doi: 10.1080/14686996.2020.1788907.

Biosynthesis of selenium nanoparticles and their protective, antioxidative effects in streptozotocin induced diabetic rats

Dabei Fan  1 , Li Li  2 , Zhizhen Li  1 , Ying Zhang  1 , Xiaojun Ma  1 , Lina Wu  1 , Haohao Zhang  1 , Feng Guo  1
Affiliations

Biosynthesis of selenium nanoparticles and their protective, antioxidative effects in streptozotocin induced diabetic rats

Dabei Fan et al. Sci Technol Adv Mater. .

Abstract

Green synthesis of selenium nanoparticles (Se NPs) was performed by mixing Hibiscus sabdariffa (roselle plant) leaf extract with the solution of selenious acid (H2SeO3) under continuous stirring conditions resulting the roselle plant secondary metabolites conjugated Se NPs. The existence of functional groups of roselle plant secondary metabolites on the surface of prepared Se NPs was confirmed by Fourier transform infrared spectroscopy (FTIR). The formation of crystalline nanoparticles with anisotropic shape was confirmed by transmission electron microscopy (TEM) images. Furthermore, we also studied anti-oxidative and protective effects of Se NPs in streptozotocin (STZ) induced diabetes rats. These STZ induced diabetic rats were daily exposed to Se NPs or/and insulin treatment and the effect of Se NPs on the factors correlated to oxidative damage in the rat testes were evaluated. The biochemical studies showed that the Se NPs are capable to enhance the serum testosterone reduction caused due to STZ induced diabetes. In addition, Se NPs can significantly reduce the oxidative stress indicators of the testicular tissue such as nitric oxide and lipid peroxidation. However, the treatment of Se NPs on the STZ induced diabetic rats increased the activities of antioxidant enzyme as well as the glutathione content in testicular tissues. Furthermore, microscopic studies revealed that the Se NPs are capable of preventing the histological damage in the testes of STZ induced diabetic rats. Altogether, these results explained the possible effects of Se NPs in attenuating oxidative damage induced by diabetes, especially in the testicular tissue.

Keywords: 100 Materials; H2SeO3; Se NPs; insulin; streptozotocin.

PubMed Disclaimer

Conflict of interest statement

No potential conflict of interest was reported by the authors.

Figures

None
Graphical abstract
Figure 1.
Figure 1.
Evolution of optical absorption signal during the synthesis of Se NPs.
Figure 2.
Figure 2.
FTIR spectra of prepared Se NPs.
Figure 3.
Figure 3.
(a) XRD pattern of Se NPs with the stick pattern of JCPDS card 06–362.
Figure 4.
Figure 4.
TEM image (a) SAED pattern (b) and DLS histogram (c) of prepared Se NPs.
Figure 5.
Figure 5.
Effect of Se NPs and/or insulin on absolute weight of testes (a) and comparative weight of testes (b) of control and STZ induced diabetic rats. p < 0.05 is considered as significant.
Figure 6.
Figure 6.
Effect of Se NPs and/or insulin on serum testosterone levels in control and STZ- diabetic rats. p < 0.05 is considered as significant.
Figure 7.
Figure 7.
Effect of insulin and Se NPs on oxidative stress markers (a) peroxidation of lipids (b) NO levels (c) glutathione levels in testes of STZ-diabetic rats and control. p < 0.05 is considered as significant.
Figure 8.
Figure 8.
Effect of insulin and Se NPs on activity of antioxidant enzymes. Levels of superoxide dismutase (a) catalase (b) glutathione peroxidase (c) and glutathione reductase (d) after exposure to Se NPs, insulin in STZ-diabetic rats and control testis. p < 0.05 is considered as significant.
Figure 9.
Figure 9.
Histological images of the testis in rats. Spermatogenic cells obtained from testes of rats were undergone for specific antibody staining against PCNA. (a) control (b) Se NPs -treated rats (c) STZ-diabetic rats, (d–f) group induced with STZ and Se NPs, rats with insulin, STZ induction and rats induced with STZ- Insulin- Se NPs, correspondingly. Scale bar is 50 µm.
See this image and copyright information in PMC

References

    1. Agarwal R, Behari JR.. Effect of selenium pretreatment in chronic mercury intoxication in rats, Bull. Environ Contam Toxicol. 2007;79:306–310. - PubMed
    1. Broome CS, McArdle F, Kyle JA, et al. An increase in selenium intake improves immune function and poliovirus handling in adults with marginal selenium status. Am J Clin Nutr. 2004;80:154–162. - PubMed
    1. Liu Y, Liu Q, Ye G, et al. Protective effects of Selenium-enriched probiotics on carbon tetrachloride-induced liver fibrosis in rats. J Agric Food Chem. 2015;63:242–249. - PubMed
    1. Ghazi Harsini S, Habibiyan M, Moeini MM, et al. Effects of dietary selenium, vitamin E, and their combination on growth, serum metabolites, and antioxidant defense system in skeletal muscle of broilers under heat stress. Biol Trace Elem Res. 2012;148:322–330. - PubMed
    1. Ghosh P, Bhattacharjee A, Basu A, et al. Attenuation of cyclophosphamide-induced pulmonary toxicity in Swiss albino mice by naphthalimide-based organoselenium compound 2-(5-selenocyanatopentyl)-benzo[de]isoquinoline 1,3-dione. Pharm Biol. 2015;53:524–532. - PubMed

LinkOut - more resources