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. 2020 Oct;46(10):956-966.
doi: 10.1007/s10886-020-01219-w. Epub 2020 Sep 17.

Determination of Cotton as a Larval Feeding Source for Lepidopteran Moths Using a Derivative from Cotton Metabolites as a Marker by LC-MS/MS Method

Jae Hak Kim  1 , Andreas Lagojda  2 , Dirk Kuehne  2 , Dieudonné Tshitenge Tshitenge  3 , Swapan Chaudhuri  4 , Daniel P Walker  5   6 , Graham Head  5
Affiliations

Determination of Cotton as a Larval Feeding Source for Lepidopteran Moths Using a Derivative from Cotton Metabolites as a Marker by LC-MS/MS Method

Jae Hak Kim et al. J Chem Ecol. 2020 Oct.

Abstract

Determination of the feeding history of polyphagous insect pests, such as noctuid moths (Lepidoptera: Noctuidae), is a critical element in developing population and resistance management strategies for such pests. To identify reliable markers for larval host plant determination and to develop simple extraction and detection methods, a metabolomics approach was implemented after acid hydrolysis of adult moth samples. We identified a derivative from cotton metabolites as a marker in adult moths that were fed cotton tissues as a larval diet, and we propose that the marker is tricycloheliocide H4 based on NMR and mass fragmentation analysis. Using this derivative from cotton metabolites as a marker, a targeted LC-MS/MS method reliably identified cotton as a larval diet in extracts of three noctuid moth species: Helicoverpa zea (cotton bollworm), Chloridea (Heliothis) virescens (tobacco budworm) and Chrysodeixis includens (soybean looper). We are using similar approaches to identify markers for other host plants including soybean.

Keywords: Cotton; Heliocides; LC-MS/MS; Metabolomics; Moths; NMR.

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