Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration

Zain Alhashem¹, Macarena Alvarez-Garcillan Portillo¹, Mint Ravinand Htun^{1

2}, Anton Gauert³, Luis Briones Montecinos⁴, Steffen Härtel⁴, Claudia Linker⁵

Affiliations

¹ Randall Centre for Cell and Molecular Biophysics, King's College, London, UK.
² The Francis Crick Institute, London, UK.
³ Department of Pediatrics Oncology and Hematology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt - Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
⁴ Program of Anatomy and Developmental Biology, BNI, CIMT, CENS, ICBM, Faculty of Medicine, University of Chile, Santiago, Chile.
⁵ Randall Centre for Cell and Molecular Biophysics, King's College, London, UK. claudia.linker@kcl.ac.uk.

PMID: 32939715
DOI: 10.1007/978-1-0716-0779-4_9

Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration

Zain Alhashem et al. Methods Mol Biol. 2021.

. 2021:2179:79-106.

doi: 10.1007/978-1-0716-0779-4_9.

Authors

Zain Alhashem¹, Macarena Alvarez-Garcillan Portillo¹, Mint Ravinand Htun^{1

2}, Anton Gauert³, Luis Briones Montecinos⁴, Steffen Härtel⁴, Claudia Linker⁵

Affiliations

¹ Randall Centre for Cell and Molecular Biophysics, King's College, London, UK.
² The Francis Crick Institute, London, UK.
³ Department of Pediatrics Oncology and Hematology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt - Universität zu Berlin, and Berlin Institute of Health, Berlin, Germany.
⁴ Program of Anatomy and Developmental Biology, BNI, CIMT, CENS, ICBM, Faculty of Medicine, University of Chile, Santiago, Chile.
⁵ Randall Centre for Cell and Molecular Biophysics, King's College, London, UK. claudia.linker@kcl.ac.uk.

PMID: 32939715
DOI: 10.1007/978-1-0716-0779-4_9

Abstract

The study of cell migration has been greatly enhanced by the development of new model systems and analysis protocols to study this process in vivo. Zebrafish embryos have been a principal protagonist because they are easily accessible, genetically tractable, and optically transparent. Neural crest cells, on the other hand, are the ideal system to study cell migration. These cells migrate extensively, using different modalities of movement and sharing many traits with metastatic cancer cells. In this chapter, we present new tools and protocols that allow the study of NC development and migration in vivo.

Keywords: Cell dissociation; Cell migration; Cell tracking; Clonal analysis; Gal4/Kalt4; In vivo imaging; Photoconversion; Tamoxifen; UAS; Zebrafish.

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Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration

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Zebrafish Neural Crest: Lessons and Tools to Study In Vivo Cell Migration

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