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. 2021 Jan;229(2):920-934.
doi: 10.1111/nph.16928. Epub 2020 Oct 14.

GmKIX8-1 regulates organ size in soybean and is the causative gene for the major seed weight QTL qSw17-1

Cuong X Nguyen  1 , Kyle J Paddock  1 , Zhanyuan Zhang  1 , Minviluz G Stacey  1
Affiliations
Free article

GmKIX8-1 regulates organ size in soybean and is the causative gene for the major seed weight QTL qSw17-1

Cuong X Nguyen et al. New Phytol. 2021 Jan.
Free article

Abstract

Seed weight is one of the most important agronomic traits in soybean for yield improvement and food production. Several quantitative trait loci (QTLs) associated with the trait have been identified in soybean. However, the genes underlying the QTLs and their functions remain largely unknown. Using forward genetic methods and CRISPR/Cas9 gene editing, we identified and characterized the role of GmKIX8-1 in the control of organ size in soybean. GmKIX8-1 belongs to a family of KIX domain-containing proteins that negatively regulate cell proliferation in plants. Consistent with this predicted function, we found that loss-of-function GmKIX8-1 mutants showed a significant increase in the size of aerial plant organs, such as seeds and leaves. Likewise, the increase in organ size is due to increased cell proliferation, rather than cell expansion, and increased expression of CYCLIN D3;1-10. Lastly, molecular analysis of soybean germplasms harboring the qSw17-1 QTL for the big-seeded phenotype indicated that reduced expression of GmKIX8-1 is the genetic basis of the qSw17-1 phenotype.

Keywords: qSw17-1; CRISPR/Cas9; KIX8; haplo-insufficient; soybean seed size.

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References

    1. Adamski NM, Anastasiou E, Eriksson S, O’Neill CM, Lenhard M. 2009. Local maternal control of seed size by KLUH/CYP78A5 dependent growth signaling. Proceedings of the National Academy of Sciences, USA 106: 20115.
    1. Baekelandt A, Pauwels L, Wang Z, Li N, De Milde L, Natran A, Vermeersch M, Li Y, Goossens A, Inzé D et al. 2018. Arabidopsis leaf flatness is regulated by ppd2 and ninja through repression of CYCLIN D3 genes. Plant Physiology 178: 217.
    1. Bailey TL, Boden M, Buske FA, Frith M, Grant CE, Clementi L, Ren J, Li WW, Noble WS. 2009. MEME SUITE: tools for motif discovery and searching. Nucleic Acids Research 37: W202-W208.
    1. Barbeira AN, Dickinson SP, Bonazzola R, Zheng J, Wheeler HE, Torres JM, Torstenson ES, Shah KP, Garcia T, Edwards TL et al. 2018. Exploring the phenotypic consequences of tissue specific gene expression variation inferred from GWAS summary statistics. Nature Communications 9: 1825.
    1. Bashandy H, Jalkanen S, Teeri TH. 2015. Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana. Plant Methods 11: 47.

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