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. 2021 Jan 20;103(2):174-183.
doi: 10.2106/JBJS.20.00385.

Assessment of Mitochondrial Dysfunction in a Murine Model of Supraspinatus Tendinopathy

Xueying Zhang  1   2 , Susumu Wada  1 , Ying Zhang  1 , Daoyun Chen  1 , Xiang-Hua Deng  1 , Scott A Rodeo  1
Affiliations

Assessment of Mitochondrial Dysfunction in a Murine Model of Supraspinatus Tendinopathy

Xueying Zhang et al. J Bone Joint Surg Am. .

Abstract

Background: The purpose of this study was to assess mitochondrial dysfunction in a murine model of supraspinatus tendinopathy.

Methods: Eighty-four mice (168 limbs) were included in the study. Supraspinatus tendinopathy was induced by inserting a microsurgical clip in the subacromial space of 63 mice bilaterally (126 limbs). Forty-two of these limbs were harvested at 4 weeks postoperatively, 42 underwent clip removal at 4 weeks after the initial procedure and were harvested at 2 weeks, and 42 underwent clip removal at 4 weeks and were harvested at 4 weeks. Forty-two limbs in the remaining 21 mice did not undergo surgical intervention and were utilized as the control group. Outcomes included biomechanical, histological, gene expression, superoxide dismutase (SOD) activity, and transmission electron microscopy (TEM) analyses.

Results: Radiographs confirmed stable clip position in the subacromial space at 4 weeks. Biomechanical testing demonstrated a 60% decrease in failure force of the supraspinatus tendons at 4 weeks compared with the control group. The failure force gradually increased at 2 and 4 weeks after clip removal. Histological analysis demonstrated inflammation surrounding the tendon with higher modified Bonar scores at 4 weeks after clip placement followed by gradual improvement following clip removal. The expression of mitochondrial-related genes was decreased at 4 weeks after clip placement and then significantly increased after clip removal. SOD activity decreased significantly at 4 weeks after clip placement but increased following clip removal. TEM images demonstrated alterations in morphology and the number of mitochondria and cristae at 4 weeks after clip placement with improvement after clip removal.

Conclusions: Mitochondrial dysfunction appears to be associated with the development of tendinopathy.

Clinical relevance: Mitochondrial protection may offer a potential strategy for delaying the development of tendinopathy and promoting tendon healing.

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Conflict of interest statement

Disclosure: The authors indicated that no external funding was received for any aspect of this work. On the Disclosure of Potential Conflicts of Interest forms, which are provided with the online version of the article, one or more of the authors checked “yes” to indicate that the author had a relevant financial relationship in the biomedical arena outside the submitted work (http://links.lww.com/JBJS/G109).

Comment in

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