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Comment
. 2020 Sep 15;117(37):22655-22656.
doi: 10.1073/pnas.2015951117.

Reply to Liu: The disassembly of the actin cytoskeleton is an early event during NETosis

Hawa Racine Thiam  1 , Siu Ling Wong  2 , Rong Qiu  3 , Mark Kittisopikul  3   4 , Amir Vahabikashi  3 , Anne E Goldman  3 , Robert D Goldman  3 , Denisa D Wagner  5   6   7 , Clare M Waterman  8
Affiliations
Comment

Reply to Liu: The disassembly of the actin cytoskeleton is an early event during NETosis

Hawa Racine Thiam et al. Proc Natl Acad Sci U S A. .
No abstract available

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Conflict of interest statement

The authors declare no competing interest.

Figures

Fig. 1.
Fig. 1.
Human blood polymorphonuclear neutrophils (Hm PMN) were plated on coverslips, stimulated with 4 μM ionomycin or vehicle control (dimethyl sulfoxide [DMSO]), and fixed at different time points after stimulation (noted in images). Cells were stained with phalloidin-Alexa fluor 694 and imaged by phase contrast (Top) and spinning-disk confocal microscopy (Bottom). Bottom images (phalloidin) represent maximum intensity projections of Z stacks (step size, 0.2 μm; total Z-depth, 5 μm [DMSO] or 8 μm [stimulated, to account for the increase in cell height after stimulation]). Double-arrow-headed line above images indicates cells that are in the presence of ionomycin. Cyan arrows indicate red blood cells (RBC). Note the presence of actin bundles in RBCs 5 and 10 min after the addition of ionomycin. Note the global decrease in phalloidin signal in Hm PMNs between before and after ionomycin stimulation in cells that shed microvesicles.
See this image and copyright information in PMC

Comment on

References

    1. Thiam H. R., et al. ., NETosis proceeds by cytoskeleton and endomembrane disassembly and PAD4-mediated chromatin decondensation and nuclear envelope rupture. Proc. Natl. Acad. Sci. U.S.A. 117, 7326–7337 (2020). - PMC - PubMed
    1. Liu M.-L., Functional actin cytoskeleton is required in early stage of NETosis induction. Proc. Natl. Acad. Sci. U.S.A. 117, 22653–22654 (2020). - PMC - PubMed
    1. Neubert E., et al. ., Chromatin swelling drives neutrophil extracellular trap release. Nat. Commun. 9, 3767 (2018). - PMC - PubMed
    1. Metzler K. D., Goosmann C., Lubojemska A., Zychlinsky A., Papayannopoulos V., A myeloperoxidase-containing complex regulates neutrophil elastase release and actin dynamics during NETosis. Cell Rep. 8, 883–896 (2014). - PMC - PubMed
    1. Sollberger G., et al. ., Gasdermin D plays a vital role in the generation of neutrophil extracellular traps. Sci. Immunol. 3, eaar6689 (2018). - PubMed

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