doi: 10.1128/jb.169.6.2876-2880.1987.
In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae
- PMID: 3294810
- PMCID: PMC212204
- DOI: 10.1128/jb.169.6.2876-2880.1987
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In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae
J Bacteriol.
1987 Jun.
Abstract
In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by covalent modification (A.J. Ninfa and B. Magasanik, Proc. Natl. Acad. Sci. USA 83:5909, 1986). Two DNA-binding sites for NTRC lie approximately 150 base pairs upstream of the nifLA promoter.
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