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. 1987 Jun;169(6):2876-80.
doi: 10.1128/jb.169.6.2876-2880.1987.

In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae

P K Wong, D Popham, J Keener, S Kustu

In vitro transcription of the nitrogen fixation regulatory operon nifLA of Klebsiella pneumoniae

P K Wong et al. J Bacteriol. 1987 Jun.

Abstract

In vitro transcription from the promoter for the nitrogen fixation regulatory operon nifLA of K. pneumoniae requires four protein fractions: the core form of RNA polymerase; NTRA, an alternate sigma factor; NTRC, an auxiliary DNA-binding protein; and NTRB, a bifunctional enzyme that controls the activity of NTRC by covalent modification (A.J. Ninfa and B. Magasanik, Proc. Natl. Acad. Sci. USA 83:5909, 1986). Two DNA-binding sites for NTRC lie approximately 150 base pairs upstream of the nifLA promoter.

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References

    1. EMBO J. 1983;2(1):39-44 - PubMed
    1. Methods Enzymol. 1980;65(1):499-560 - PubMed
    1. Nature. 1980 Jul 10;286(5769):128-32 - PubMed
    1. J Bacteriol. 1980 Oct;144(1):210-6 - PubMed
    1. J Bacteriol. 1980 Nov;144(2):744-51 - PubMed

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