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. 2020 Dec;97(6):1880-1885.
doi: 10.1111/jfb.14551. Epub 2020 Oct 5.

A comprehensive description of oocyte developmental stages in Pacific halibut, Hippoglossus stenolepis

Teresa Fish  1   2 , Nathan Wolf  1 , Bradley P Harris  1 , Josep V Planas  2
Affiliations

A comprehensive description of oocyte developmental stages in Pacific halibut, Hippoglossus stenolepis

Teresa Fish et al. J Fish Biol. 2020 Dec.

Abstract

Accurate characterization of oocyte development is essential to understanding foundational aspects of reproductive biology and successful management of Pacific halibut (Hippoglossus stenolepis). Here this study provides complete histological descriptions for eight oocyte developmental stages in addition to postovulatory follicles and demonstrates the potential for oocyte size frequency distribution to act as a proxy for ovarian developmental stage and future maturity assessments. Importantly, it provides the first histological evidence that Pacific halibut have a group-synchronous ovarian developmental pattern with determinate fecundity and support for their batch-spawning strategy.

Keywords: Pacific halibut; developmental stage; histology; maturity; oocyte; reproduction.

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Conflict of interest statement

All authors declare that they have no conflict of interest.

Figures

FIGURE 1
FIGURE 1
Description of changes in Pacific halibut mean oocyte diameters with (a) developmental stage and (b) oocyte size distribution with individual fish developmental stage. Vertical broken lines separate the growth stages at the midpoint between mean oocyte developmental stage diameters. In (a), mean oocyte diameters are indicated by black circles, one‐ and two standard deviations are indicated by wide black and grey bars, respectively; and minimum and maximum values are indicated by narrow grey bars, with different letters (a–f) indicating statistical significance (P < 0.025) among groups. In (b) the distribution of oocyte diameters is represented as the proportional occupancy of ln(oocyte area) for each of the observed female developmental stages (defined as the most advanced stage of oocyte present in the sample population; PGpn, PGca, etc.), with n indicating the total number of oocytes measured from a sub‐set of females (number of individual fish shown in parentheses)
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