Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Jun;84(11):3648-52.
doi: 10.1073/pnas.84.11.3648.

TUF, the yeast DNA-binding factor specific for UASrpg upstream activating sequences: identification of the protein and its DNA-binding domain

J Huet, A Sentenac

TUF, the yeast DNA-binding factor specific for UASrpg upstream activating sequences: identification of the protein and its DNA-binding domain

J Huet et al. Proc Natl Acad Sci U S A. 1987 Jun.

Abstract

The factor TUF interacts specifically with RPG or HOMOL1 sequences, which are present upstream of many genes coding for the yeast translational apparatus. Here we present evidence that the RPG and HOMOL1 motifs are variants of a consensus UASrpg (upstream activating sequence) recognized by the same factor. Factor TUF was identified by using two highly selective methods. The DNA-protein complex was isolated by pore-limit electrophoresis in polyacrylamide gradient gels and found to contain a single polypeptide of 150 kDa. In a two-step protein-blotting/nuclease-protection ("footprinting") procedure, the same 150-kDa polypeptide blotted on nitrocellulose exhibited the same specific DNA-binding properties as TUF factor. A 50-kDa DNA-binding domain of TUF was isolated by selective proteolysis. This suggests a bipolarization of the TUF protein, with distinct functional domains.

PubMed Disclaimer

References

    1. EMBO J. 1985 Dec 16;4(13A):3539-47 - PubMed
    1. Nature. 1986 Sep 11-17;323(6084):176-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Oct;82(20):6741-4 - PubMed
    1. Science. 1986 Oct 3;234(4772):47-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Dec;81(24):7865-9 - PubMed