The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot
- PMID: 32959265
- DOI: 10.1007/978-1-0716-0795-4_30
The Quantification of Antigen-Specific T Cells by IFN-γ ELISpot
Abstract
The enzyme-linked immune absorbent spot (ELISpot) assay allows for the quantification of the number of cells producing a particular secreted analyte. As T lymphocytes secrete cytokines such as interferon (IFN)-γ upon binding of the T cell receptor with its cognate antigen epitope, IFN-γ ELISpot allows for the measurement of antigen-specific T cells in an immune sample. Immune cells are isolated from the vaccinated subject and incubated with the epitope/antigen of interest on polyvinylidene difluoride (PVDF)-lined microplates precoated with a capture antibody to IFN-γ. Cytokine spots are then detected utilizing an IFN-γ-specific detection antibody and an enzyme-linked conjugate. Here, we describe the quantification of OVA-specific CD8 and CD4 T cells from mouse splenocytes to measure vaccine-induced cellular responses.
Keywords: ELISpot; Interferon-γ; Ovalbumin; T lymphocyte; Vaccines.
Similar articles
-
The Ex Vivo IFN-γ Enzyme-Linked Immunospot (ELISpot) Assay.Methods Mol Biol. 2015;1325:197-205. doi: 10.1007/978-1-4939-2815-6_16. Methods Mol Biol. 2015. PMID: 26450390
-
CTL ELISPOT Assay and T Cell Detection.Methods Mol Biol. 2021;2325:65-77. doi: 10.1007/978-1-0716-1507-2_5. Methods Mol Biol. 2021. PMID: 34053051
-
CTL ELISPOT assay.Methods Mol Biol. 2014;1186:75-86. doi: 10.1007/978-1-4939-1158-5_6. Methods Mol Biol. 2014. PMID: 25149304
-
ELISpot for measuring human immune responses to vaccines.Expert Rev Vaccines. 2011 Mar;10(3):299-306. doi: 10.1586/erv.10.169. Expert Rev Vaccines. 2011. PMID: 21434798 Free PMC article. Review.
-
ELISpot assay and prediction of organ transplant rejection.Int J Immunogenet. 2022 Feb;49(1):39-45. doi: 10.1111/iji.12565. Epub 2021 Dec 15. Int J Immunogenet. 2022. PMID: 34910357 Review.
Cited by
-
Evaluation of Adjuvant Activity and Bio-Distribution of Archaeosomes Prepared Using Microfluidic Technology.Pharmaceutics. 2022 Oct 26;14(11):2291. doi: 10.3390/pharmaceutics14112291. Pharmaceutics. 2022. PMID: 36365110 Free PMC article.
-
The screening, identification, design and clinical application of tumor-specific neoantigens for TCR-T cells.Mol Cancer. 2023 Aug 30;22(1):141. doi: 10.1186/s12943-023-01844-5. Mol Cancer. 2023. PMID: 37649123 Free PMC article. Review.
-
Sulfated Lactosyl Archaeol Archaeosome-Adjuvanted Vaccine Formulations Targeting Rabbit Hemorrhagic Disease Virus Are Immunogenic and Efficacious.Vaccines (Basel). 2023 May 31;11(6):1043. doi: 10.3390/vaccines11061043. Vaccines (Basel). 2023. PMID: 37376432 Free PMC article.
-
Sulfated Lactosyl Archaeol Archaeosomes Synergize with Poly(I:C) to Enhance the Immunogenicity and Efficacy of a Synthetic Long Peptide-Based Vaccine in a Melanoma Tumor Model.Pharmaceutics. 2021 Feb 12;13(2):257. doi: 10.3390/pharmaceutics13020257. Pharmaceutics. 2021. PMID: 33673382 Free PMC article.
-
Homologous Sequential Immunization Using Salmonella Oral Administration Followed by an Intranasal Boost with Ferritin-Based Nanoparticles Enhanced the Humoral Immune Response against H1N1 Influenza Virus.Microbiol Spectr. 2023 Jun 15;11(3):e0010223. doi: 10.1128/spectrum.00102-23. Epub 2023 May 8. Microbiol Spectr. 2023. PMID: 37154735 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
