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. 2020 Sep 19;9(9):301.
doi: 10.3390/biology9090301.

Can Chitin and Chitosan Replace the Lichen Evernia prunastri for Environmental Biomonitoring of Cu and Zn Air Contamination?

Stefano Loppi  1 , Andrea Vannini  1 , Fabrizio Monaci  1 , Daniel Dagodzo  1 , Felix Blind  2 , Michael Erler  2 , Stefan Fränzle  2
Affiliations

Can Chitin and Chitosan Replace the Lichen Evernia prunastri for Environmental Biomonitoring of Cu and Zn Air Contamination?

Stefano Loppi et al. Biology (Basel). .

Abstract

This study compared the ability of the lichen Evernia prunastri, chitin and chitosan to take up Cu2+ and Zn2+. It was hypothesized that chitin and chitosan have an accumulation capacity comparable to the lichen, so that these biopolymers could replace the use of E. prunastri for effective biomonitoring of Cu and Zn air pollution. Samples of the lichen E. prunastri, as well as chitin (from shrimps) and chitosan (from crabs), were incubated with Cu and Zn solutions at concentrations of 0 (control), 10, 25, 50, 75, and 100 µM and analyzed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Metal concentrations accumulated by lichen, chitin and chitosan samples were strongly and linearly correlated with the concentrations in the treatment solutions. The lichen always showed significantly higher accumulation values compared to chitin and chitosan, which showed similar accumulation features. The outcomes of this study confirmed the great effectiveness of the lichen Evernia prunastri for environmental biomonitoring and showed that chitin and chitosan have a lower accumulation capacity, thus suggesting that although these biopolymers have the potential for replacing E. prunastri in polluted areas, their suitability may be limited in areas with intermediate or low pollution levels.

Keywords: Cu; Zn; bioaccumulation; biopolymers; biosorption; ion exchange.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Copper and zinc concentrations (µg/g) in lichen, chitin, and chitosan samples after incubation with Cu (left) and Zn (right) solutions at the concentration 0 (control), 10, 25, 50, 75, and 100 µM. The grey area indicates the IC95 confidence interval.
Figure 2
Figure 2
Copper and zinc accumulation (ratio between treated and control value ± SE) in lichen, chitin, and chitosan samples after incubation with Cu (up) and Zn (down) solutions at the concentration of 10, 25, 50, 75, and 100 µM. Different letters (a, b) indicate statistical significant differences between matrices (p < 0.05).
Figure 2
Figure 2
Copper and zinc accumulation (ratio between treated and control value ± SE) in lichen, chitin, and chitosan samples after incubation with Cu (up) and Zn (down) solutions at the concentration of 10, 25, 50, 75, and 100 µM. Different letters (a, b) indicate statistical significant differences between matrices (p < 0.05).
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