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Review
. 2020 Sep 17:36:33.
doi: 10.1186/s42826-020-00066-w. eCollection 2020.

Cryopreservation of mouse resources

Toru Takeo  1 , Satohiro Nakao  1 , Yoshiko Nakagawa  1 , Jorge M Sztein  1 , Naomi Nakagata  2
Affiliations
Review

Cryopreservation of mouse resources

Toru Takeo et al. Lab Anim Res. .

Abstract

The cryopreservation of sperm and embryos is useful to efficiently archive valuable resources of genetically engineered mice. Till date, more than 60,000 strains of genetically engineered mice have been archived in mouse banks worldwide. Researchers can request for the archived mouse strains for their research projects. The research infrastructure of mouse banks improves the availability of mouse resources, the productivity of research projects, and the reproducibility of animal experiments. Our research team manages the mouse bank at the Center for Animal Resources and Development in Kumamoto University and continuously develops new techniques in mouse reproductive technology to efficiently improve the system of mouse banking. In this review, we introduce the activities of mouse banks and the latest techniques used in mouse reproductive technology.

Keywords: Cold storage; Cryopreservation; Embryo; Genetically engineered mice; Hands-on workshop; In vitro fertilization; Mouse bank; Reproductive technology; Sperm.

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Conflict of interest statement

Competing interestsThere is no competing interest associated with this article.

Figures

Fig. 1
Fig. 1
Application of advanced mouse reproductive technology in a mouse bank
See this image and copyright information in PMC

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