Melatonin ingestion before intradialytic exercise improves immune responses in hemodialysis patients

Houssem Marzougui^{1

2}, Omar Hammouda^{3

4}, Imen Ben Dhia^{2

5}, Rami Maaloul^{1

2}, Ikram Agrebi^{6

7}, Hanen Chaker^{6

7}, Khaoula Kammoun^{6

7}, Mohamed Ben Hmida^{6

7}, Fatma Ayadi^{1

8}, Choumous Kallel⁹, Tarak Driss¹⁰, Mouna Turki^{1

8}, Hatem Masmoudi^{11

12}, Hend Hachicha^{11

12}

Affiliations

¹ Research Laboratory, Molecular Bases of Human Pathology, LR19ES13, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
² High Institute of Sport and Physical Education, University of Sfax, Sfax, Tunisia.
³ Research Laboratory, Molecular Bases of Human Pathology, LR19ES13, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia. hammouda.o@parisnanterre.fr.
⁴ Interdisciplinary Laboratory in Neurosciences, Physiology and Psychology: Physical Activity, Health and Learning (LINP2), UFR STAPS, UPL, Paris Nanterre University, 92000, Nanterre, France. hammouda.o@parisnanterre.fr.
⁵ The Research Unit of the Assessment of Musculoskeletal Disorders, University of Sfax, UR12ES18, Sfax, Tunisia.
⁶ Nephrology Department, Hedi Chaker University Hospital, University of Sfax, Sfax, Tunisia.
⁷ Research Laboratory of Renal Pathology LR19ES11, Faculty of Medicine, University of Sfax, Sfax, Tunisia.
⁸ Biochemistry Laboratory, Habib Bourguiba University Hospital, University of Sfax, Sfax, Tunisia.
⁹ Laboratory of Hematology, Habib Bourguiba University Hospital, University of Sfax, Sfax, Tunisia.
¹⁰ Interdisciplinary Laboratory in Neurosciences, Physiology and Psychology: Physical Activity, Health and Learning (LINP2), UFR STAPS, UPL, Paris Nanterre University, 92000, Nanterre, France.
¹¹ Immunology Department, Habib Bourguiba University Hospital, University of Sfax, Sfax, Tunisia.
¹² Research Labratory, Autoimmunity Cancer and Immunogenetics, LR18/SP12, Habib Bourguiba University Hospital, University of Sfax, Sfax, Tunisia.

PMID: 32965623
DOI: 10.1007/s11255-020-02643-3

Randomized Controlled Trial

Melatonin ingestion before intradialytic exercise improves immune responses in hemodialysis patients

Houssem Marzougui et al. Int Urol Nephrol. 2021 Mar.

. 2021 Mar;53(3):553-562.

doi: 10.1007/s11255-020-02643-3. Epub 2020 Sep 23.

Authors

Affiliations

¹ Research Laboratory, Molecular Bases of Human Pathology, LR19ES13, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia.
² High Institute of Sport and Physical Education, University of Sfax, Sfax, Tunisia.
³ Research Laboratory, Molecular Bases of Human Pathology, LR19ES13, Faculty of Medicine of Sfax, University of Sfax, Sfax, Tunisia. hammouda.o@parisnanterre.fr.
⁴ Interdisciplinary Laboratory in Neurosciences, Physiology and Psychology: Physical Activity, Health and Learning (LINP2), UFR STAPS, UPL, Paris Nanterre University, 92000, Nanterre, France. hammouda.o@parisnanterre.fr.
⁵ The Research Unit of the Assessment of Musculoskeletal Disorders, University of Sfax, UR12ES18, Sfax, Tunisia.
⁶ Nephrology Department, Hedi Chaker University Hospital, University of Sfax, Sfax, Tunisia.
⁷ Research Laboratory of Renal Pathology LR19ES11, Faculty of Medicine, University of Sfax, Sfax, Tunisia.
⁸ Biochemistry Laboratory, Habib Bourguiba University Hospital, University of Sfax, Sfax, Tunisia.
⁹ Laboratory of Hematology, Habib Bourguiba University Hospital, University of Sfax, Sfax, Tunisia.
¹⁰ Interdisciplinary Laboratory in Neurosciences, Physiology and Psychology: Physical Activity, Health and Learning (LINP2), UFR STAPS, UPL, Paris Nanterre University, 92000, Nanterre, France.
¹¹ Immunology Department, Habib Bourguiba University Hospital, University of Sfax, Sfax, Tunisia.
¹² Research Labratory, Autoimmunity Cancer and Immunogenetics, LR18/SP12, Habib Bourguiba University Hospital, University of Sfax, Sfax, Tunisia.

PMID: 32965623
DOI: 10.1007/s11255-020-02643-3

Abstract

Purpose: The present study aimed to investigate the effects of melatonin (MEL) intake on systemic inflammation and immune responses during intradialytic exercise.

Methods: Thirteen hemodialysis (HD) patients volunteered to participate in the current randomized-crossover study. Immunological responses were monitored in four HD sessions at different conditions: [Exercise (EX) + MEL], [EX + Placebo (PLA)], [Control (CON) + MEL] and [CON + PLA]. MEL (3 mg) or PLA was ingested 1 h before starting exercise or the equivalent time in CON condition. During all sessions, peripheral blood samples were collected to assess c-reactive protein, complete blood count, and immune cells phenotypes before HD (T0), immediately after exercise (T1) and 1 h after exercise (T2) or at corresponding times in the CON condition.

Results: HD therapy induced a significant decrease in natural killer (NK) (p = 0.001, d = 0.85; p < 0.001, d = 1.19, respectively) and CD8⁺ T-lymphocytes rates (p = 0.001, d = 0.57; p < 0.001, d = 0.75, respectively) at T1 and T2 compared to T0. MEL intake prevented the decrease in NK and CD8⁺ T-lymphocytes, increased the proportion of CD4⁺ T-lymphocytes at T1 and T2 compared to T0 (p = 0.002, d = 1.18; p = 0.001, d = 1.04, respectively) and decreased the proportion of CD14⁺⁺CD16⁺ Monocytes at T2 compared to T0 (p = 0.02, d = 1.57) in peripheral blood during HD therapy. Similar results were found in [EX + MEL] and [EX + PLA] conditions.

Conclusion: This pilot study provides the first evidence that MEL intake alone or associated with intradialytic exercise displays potential immunoregulatory and anti-inflammatory effects. The combination of MEL with intradialytic exercise may be an appropriate anti-inflammatory therapy for HD patients.

Keywords: Immune cells; Inflammation; Intradialytic exercise; Melatonin; Renal failure.

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References

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Melatonin ingestion before intradialytic exercise improves immune responses in hemodialysis patients

Affiliations

Melatonin ingestion before intradialytic exercise improves immune responses in hemodialysis patients

Authors

Affiliations

Abstract

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LinkOut - more resources

Full Text Sources

Medical

Research Materials