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. 2020 Oct 20;56(83):12526-12529.
doi: 10.1039/d0cc05540a.

CRISPR-Cas12a trans-cleaves DNA G-quadruplexes

Ying Li  1 , Tao Li  1 , Bi-Feng Liu  2 , Rui Hu  1 , Jiang Zhu  3 , Ting He  3 , Xin Zhou  1 , Conggang Li  1 , Yunhuang Yang  1 , Maili Liu  1
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CRISPR-Cas12a trans-cleaves DNA G-quadruplexes

Ying Li et al. Chem Commun (Camb). .

Abstract

We for the first time report that the activated CRISPR-Cas12a system trans-cleaves DNA G-quadruplexes (G4). The cleavage activity on human telomere G4 and TBA G4 was investigated and verified by FRET, CD, gel electrophoresis and NMR. We believe that this finding will pave a new avenue for advancing the applications of CRISPR-Cas12a and G4 in biosensing and biochemistry.

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