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. 1987 Jan 15;241(2):573-81.
doi: 10.1042/bj2410573.

Purification and kinetic properties of pyruvate kinase isoenzymes of Salmonella typhimurium

C Garcia-Olalla, A Garrido-Pertierra

Purification and kinetic properties of pyruvate kinase isoenzymes of Salmonella typhimurium

C Garcia-Olalla et al. Biochem J. .

Abstract

Two forms of pyruvate kinase (ATP: pyruvate 2-O-phosphotransferase, EC 2.7.1.40) present in Salmonella typhimurium were purified to homogeneity from the same cultures by (NH4)2SO4 fractionation and gel filtration, anion-exchange and affinity chromatography. Mr values, subunit structure, amino acid composition and activity and stability conditions were determined for the two forms. Kinetic and regulatory properties of the two purified isoenzymes were studied.

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