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. 2019 Oct 29;1(10):e000067.
doi: 10.1099/acmi.0.000067. eCollection 2019.

High-quality draft genome sequences of Pseudomonas monteilii DSM 14164T, Pseudomonas mosselii DSM 17497T, Pseudomonas plecoglossicida DSM 15088T, Pseudomonas taiwanensis DSM 21245T and Pseudomonas vranovensis DSM 16006T: taxonomic considerations

Arantxa Peña  1 , Antonio Busquets  1 , Margarita Gomila  1 , Magdalena Mulet  1 , Rosa M Gomila  2 , Elena Garcia-Valdes  1   3 , T B K Reddy  4 , Marcel Huntemann  4 , Neha Varghese  4 , Natalia Ivanova  4 , I-Min Chen  4 , Markus Göker  5 , Tanja Woyke  4 , Hans-Peter Klenk  6 , Nikos Kyrpides  4 , Jorge Lalucat  1   3
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High-quality draft genome sequences of Pseudomonas monteilii DSM 14164T, Pseudomonas mosselii DSM 17497T, Pseudomonas plecoglossicida DSM 15088T, Pseudomonas taiwanensis DSM 21245T and Pseudomonas vranovensis DSM 16006T: taxonomic considerations

Arantxa Peña et al. Access Microbiol. .

Abstract

Pseudomonas is the bacterial genus of Gram-negative bacteria with the highest number of recognized species. It is divided phylogenetically into three lineages and at least 11 groups of species. The Pseudomonas putida group of species is one of the most versatile and best studied. It comprises 15 species with validly published names. As a part of the Genomic Encyclopedia of Bacteria and Archaea (GEBA) project, we present the genome sequences of the type strains of five species included in this group: Pseudomonas monteilii (DSM 14164T), Pseudomonas mosselii (DSM 17497T), Pseudomonas plecoglossicida (DSM 15088T), Pseudomonas taiwanensis (DSM 21245T) and Pseudomonas vranovensis (DSM 16006T). These strains represent species of environmental and also of clinical interest due to their pathogenic properties against humans and animals. Some strains of these species promote plant growth or act as plant pathogens. Their genome sizes are among the largest in the group, ranging from 5.3 to 6.3 Mbp. In addition, the genome sequences of the type strains in the Pseudomonas taxonomy were analysed via genome-wide taxonomic comparisons of ANIb, gANI and GGDC values among 130 Pseudomonas strains classified within the group. The results demonstrate that at least 36 genomic species can be delineated within the P. putida phylogenetic group of species.

Keywords: Genomic Encyclopedia of Bacteria and Archaea (GEBA); P. monteilii; P. mosselii; P. plecoglossicida; P. taiwanensis; P. vranovensis; Thousand Microbial Genomes Project (KMG I); taxonomy; type strains.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Fig. 1.
Fig. 1.
UPGMA dendrogram of the ANIb similarities of the genomes studied. The dotted line represents the recommended 95 % species threshold. Species type strains are labelled in bold. Groupings of strains using species cut-offs of 95 % are coloured and numbered.
Fig. 2.
Fig. 2.
UPGMA dendrogram of the genome-to-genome distances of the genomes studied. The dotted line represents the recommended 70 % species threshold. Species type strains are labelled in bold. Groupings of strains using species cut-offs of 70 % are coloured and numbered.
See this image and copyright information in PMC

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