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. 2021:2221:15-28.
doi: 10.1007/978-1-0716-0989-7_2.

Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Roberto Narcisi  1 , Wendy J L M Koevoet  2 , Gergo J V M van Osch  3   2
Affiliations

Expansion and Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells

Roberto Narcisi et al. Methods Mol Biol. 2021.

Abstract

Human bone marrow-derived mesenchymal stem/stromal cells (BM-MSC) are adult multipotent progenitor cells that can be isolated from bone marrow. BM-MSCs have the ability to be expanded and differentiated into the chondrogenic lineage in vitro. Here we describe a standardized method to expand and chondrogenically differentiate human BM-MSCs, highlighting how to overcome technical challenges and indicating the most common readout parameters to evaluate the chondrogenic differentiation capacity.

Keywords: Cartilage; Chondrogenesis; Mesenchymal stem cells; Regenerative medicine; Tissue engineering.

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