Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 May;160(2):187-94.
doi: 10.1016/s0005-8165(77)80024-3.

Immunomorphologic methods in routine pathology. Application of immunofluorescence and the unlabeled antibody-enzyme (peroxidase-antiperoxidase) technique to formalin fixed paraffin embedded kidney biopsies

H Denk, G Syré, E Weirich

Immunomorphologic methods in routine pathology. Application of immunofluorescence and the unlabeled antibody-enzyme (peroxidase-antiperoxidase) technique to formalin fixed paraffin embedded kidney biopsies

H Denk et al. Beitr Pathol. 1977 May.

Abstract

Immunofluorescence and the unlabeled antibody enzyme (peroxidase - antiperoxidase) method were applied to formalin fixed conventionally paraffin-embedded kidney biopsy material, and optimal working conditions are reported. Both methods were suitable for the demonstration of immunoglobulin and complement deposits within glomerula. They were equally sensitive on dewaxed pronase-treated sections. Pronase treatment decreased nonspecific background fluorescence and increased the sensitivity of both immunomorphologic techniques, possibly by enhancing antigenicity.

PubMed Disclaimer