Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1977 Jun 15;164(3):653-8.
doi: 10.1042/bj1640653.

Chitin biosynthesis in protoplasts and subcellular fractions of Aspergillus fumigatus

D B Archer

Chitin biosynthesis in protoplasts and subcellular fractions of Aspergillus fumigatus

D B Archer. Biochem J. .

Abstract

The biosynthesis of chitin has been obtained in broken mycelia and protoplasts of the fungus Aspergillus fumigatus. The specific activity of chitin synthase (EC 2.4.1.16) in a membrane preparation from protoplasts derived from the hyphal tips of A. fumigatus was 26.8-fold greater than that of the chitin synthase in broken mycelia, indicating that the active chitin synthase is located primarily in a membrane-bound site at the hyphal tip. Polyoxin D was a potent competitive inhibitor of the enzyme, having Ki 5.2 +/- 0.8 micron with respect to the natural substrate UDP-N-acetyl-D-glucosamine, which has Km 1.58 mM.

PubMed Disclaimer

References

    1. J Biol Chem. 1957 Oct;228(2):729-42 - PubMed
    1. J Bacteriol. 1970 Oct;104(1):189-96 - PubMed
    1. Antonie Van Leeuwenhoek. 1976;42(3):261-76 - PubMed
    1. J Am Chem Soc. 1969 Dec 17;91(26):7490-505 - PubMed
    1. Biochemistry. 1966 Apr;5(4):1149-54 - PubMed

MeSH terms