First case of IMP-15-producing Pseudomonas aeruginosa in Switzerland: Challenging laboratory diagnosis using whole genome sequencing (WGS)

doi:10.1016/j.idcr.2020.e00933

Case Reports

. 2020 Sep 2:22:e00933.

doi: 10.1016/j.idcr.2020.e00933. eCollection 2020.

First case of IMP-15-producing Pseudomonas aeruginosa in Switzerland: Challenging laboratory diagnosis using whole genome sequencing (WGS)

Daniel Goldenberger¹, Danielle Vuichard-Gysin², Kathrin Herzog³, Vladimira Hinić¹, Helena Seth-Smith^{1

4}, Adrian Egli^{1

4}

Affiliations

¹ Division of Clinical Bacteriology and Mycology, University Hospital Basel and University of Basel, Basel, Switzerland.
² Division of Infectious Diseases and Hospital Epidemiology, Thurgau Hospital Group, Muensterlingen and Frauenfeld, Switzerland.
³ Laboratory for Clinical Microbiology, Thurgau Hospital Group, Muensterlingen and Frauenfeld, Switzerland.
⁴ Applied Microbiology Research, Department of Biomedicine, University of Basel, Switzerland.

PMID: 32983889
PMCID: PMC7494785
DOI: 10.1016/j.idcr.2020.e00933

Case Reports

First case of IMP-15-producing Pseudomonas aeruginosa in Switzerland: Challenging laboratory diagnosis using whole genome sequencing (WGS)

Daniel Goldenberger et al. IDCases. 2020.

. 2020 Sep 2:22:e00933.

doi: 10.1016/j.idcr.2020.e00933. eCollection 2020.

Authors

Daniel Goldenberger¹, Danielle Vuichard-Gysin², Kathrin Herzog³, Vladimira Hinić¹, Helena Seth-Smith^{1

4}, Adrian Egli^{1

4}

Affiliations

¹ Division of Clinical Bacteriology and Mycology, University Hospital Basel and University of Basel, Basel, Switzerland.
² Division of Infectious Diseases and Hospital Epidemiology, Thurgau Hospital Group, Muensterlingen and Frauenfeld, Switzerland.
³ Laboratory for Clinical Microbiology, Thurgau Hospital Group, Muensterlingen and Frauenfeld, Switzerland.
⁴ Applied Microbiology Research, Department of Biomedicine, University of Basel, Switzerland.

PMID: 32983889
PMCID: PMC7494785
DOI: 10.1016/j.idcr.2020.e00933

Abstract

We report on the first case of multiresistant Pseudomonas aeruginosa harbouring the metallo-β-lactamase IMP-15 isolated in Switzerland from a patient repatriated from Cambodia. The laboratory diagnosis of IMP-15 was hampered by two negative tests for carbapenemase detection. The carbapenemase gene was subsequently detected by whole genome sequencing and the isolate further characterised by various phenotypic and genotypic analyses.

Keywords: Cabapenemase-producing Pseudomonas aeruginosa; Cambodia; Metallo-β-lactamase; Switzerland; Whole genome sequencing (WGS); blaIMP-15.

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Conflict of interest statement

The authors report no declarations of interest.

Figures

**Fig. 1**
*bla*_IMP-15 containing 10Kb region. The region is flanked by 1355bp inverted repeats covering genes encoding tyrosine recombinase XerD. The *bla*_IMP-15 is shown in red, along with other annotated genes in the region in forward or reverse frames. Matches to database sequences are shown below in grey. Figure was adapted from data visualized in Artemis [5].

See this image and copyright information in PMC

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Spottiswoode N, Hao S, Sanchez-Guerrero E, Detweiler AM, Mekonen H, Neff N, Macmillan H, Schwartz BS, Engel J, DeRisi JL, Miller SA, Langelier CR. Spottiswoode N, et al. Front Cell Infect Microbiol. 2023 Aug 30;13:1241608. doi: 10.3389/fcimb.2023.1241608. eCollection 2023. Front Cell Infect Microbiol. 2023. PMID: 37712060 Free PMC article.

References

1. Hong D.J., Bae I.K., Jang I.H., Jeong S.H., Kang H.K., Lee K. Epidemiology and characteristics of metallo-beta-lactamase-producing Pseudomonas aeruginosa. Infect Chemother. 2015;47(2):81–97. - PMC - PubMed
1. Watanabe M., Iyobe S., Inoue M., Mitsuhashi S. Transferable imipenem resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1991;35(1):147–151. - PMC - PubMed
1. Wick R.R., Judd L.M., Gorrie C.L., Holt K.E. Completing bacterial genome assemblies with multiplex MinION sequencing. Microb Genom. 2017;3(10):e000132. - PMC - PubMed
1. Prokka Seemann T. Rapid prokaryotic genome annotation. Bioinformatics. 2014;30(14):2068–2069. - PubMed
1. Carver T., Harris S.R., Berriman M., Parkhill J., McQuillan J.A. Artemis: an integrated platform for visualization and analysis of high-throughput sequence-based experimental data. Bioinformatics. 2012;28(4):464–469. - PMC - PubMed

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[1] Hong D.J., Bae I.K., Jang I.H., Jeong S.H., Kang H.K., Lee K. Epidemiology and characteristics of metallo-beta-lactamase-producing Pseudomonas aeruginosa. Infect Chemother. 2015;47(2):81–97. - PMC - PubMed

[2] Hong D.J., Bae I.K., Jang I.H., Jeong S.H., Kang H.K., Lee K. Epidemiology and characteristics of metallo-beta-lactamase-producing Pseudomonas aeruginosa. Infect Chemother. 2015;47(2):81–97. - PMC - PubMed

[3] Watanabe M., Iyobe S., Inoue M., Mitsuhashi S. Transferable imipenem resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1991;35(1):147–151. - PMC - PubMed

[4] Watanabe M., Iyobe S., Inoue M., Mitsuhashi S. Transferable imipenem resistance in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1991;35(1):147–151. - PMC - PubMed

[5] Wick R.R., Judd L.M., Gorrie C.L., Holt K.E. Completing bacterial genome assemblies with multiplex MinION sequencing. Microb Genom. 2017;3(10):e000132. - PMC - PubMed

[6] Wick R.R., Judd L.M., Gorrie C.L., Holt K.E. Completing bacterial genome assemblies with multiplex MinION sequencing. Microb Genom. 2017;3(10):e000132. - PMC - PubMed

[7] Prokka Seemann T. Rapid prokaryotic genome annotation. Bioinformatics. 2014;30(14):2068–2069. - PubMed

[8] Prokka Seemann T. Rapid prokaryotic genome annotation. Bioinformatics. 2014;30(14):2068–2069. - PubMed

[9] Carver T., Harris S.R., Berriman M., Parkhill J., McQuillan J.A. Artemis: an integrated platform for visualization and analysis of high-throughput sequence-based experimental data. Bioinformatics. 2012;28(4):464–469. - PMC - PubMed

[10] Carver T., Harris S.R., Berriman M., Parkhill J., McQuillan J.A. Artemis: an integrated platform for visualization and analysis of high-throughput sequence-based experimental data. Bioinformatics. 2012;28(4):464–469. - PMC - PubMed

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First case of IMP-15-producing Pseudomonas aeruginosa in Switzerland: Challenging laboratory diagnosis using whole genome sequencing (WGS)

Affiliations

First case of IMP-15-producing Pseudomonas aeruginosa in Switzerland: Challenging laboratory diagnosis using whole genome sequencing (WGS)

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Conflict of interest statement

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Abstract

Conflict of interest statement

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