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. 2020 Sep 28;10(1):25.
doi: 10.1186/s12348-020-00216-0.

Characterization of Pseudomonas aeruginosa isolates from patients with endophthalmitis using conventional microbiologic techniques and whole genome sequencing

Jesse D Sengillo  1 , Jacob Duker  1 , Maribel Hernandez  1 , Jorge Maestre  1 , Daniela Reyes-Capo  1 , Annika Patel  1 , Arjun Watane  1 , Nimesh A Patel  1 , Nicolas A Yannuzzi  1 , Darlene Miller  1 , Harry W Flynn Jr  2
Affiliations

Characterization of Pseudomonas aeruginosa isolates from patients with endophthalmitis using conventional microbiologic techniques and whole genome sequencing

Jesse D Sengillo et al. J Ophthalmic Inflamm Infect. .

Abstract

Purpose: To demonstrate antibiotic susceptibility and genomic virulence factor profiles of Pseudomonas aeruginosa isolates from patients with culture-confirmed endophthalmitis.

Methods: Clinical isolates from patients diagnosed with pseudomonas endophthalmitis were included. Laboratory antibiotic susceptibility testing and whole genome sequencing was performed on all isolates.

Results: In the current study, 8 patients had vitreous culture-confirmed endophthalmitis due to P. aeruginosa. All isolates were multi-drug resistant but sensitive to ceftazidime and each fluoroquinolone tested. Whole genome sequencing revealed a total of 179 unique genes. The most common type of virulence genes included those involved in adherence and the secretion system. Seven of 8 (88%) isolates were of the cytoinvasive phenotype (exoST) and no isolates contained exoU.

Conclusions: P. aeruginosa associated endophthalmitis is often multi-drug resistant and demonstrates a variety of virulence factors with those involved in adherence and the secretion system being the most common.

Keywords: Endophthalmitis; Pseudomonas aeruginosa; Whole genome sequencing.

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Conflict of interest statement

The authors declare that they have no competing interests.

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