Exosomes as Part of the Human Adipose-Derived Stem Cells Secretome- Opening New Perspectives for Cell-Free Regenerative Applications

doi:10.1007/5584_2020_588

Review

. 2021:1312:139-163.

doi: 10.1007/5584_2020_588.

Exosomes as Part of the Human Adipose-Derived Stem Cells Secretome- Opening New Perspectives for Cell-Free Regenerative Applications

Sorina Dinescu^{1

2}, Alexandra Dobranici^#³, Ramona Tecucianu^#³, Aida Selaru^{3

4}, Roxana Balahura^{3

4}, Simona Ignat³, Marieta Costache^{3

5}

Affiliations

¹ Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania. sorina.dinescu@bio.unibuc.ro.
² The Research Institute of the University of Bucharest, Bucharest, Romania. sorina.dinescu@bio.unibuc.ro.
³ Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania.
⁴ Victor Babes National Institute of Pathology, Bucharest, Romania.
⁵ The Research Institute of the University of Bucharest, Bucharest, Romania.

^# Contributed equally.

PMID: 32986128
DOI: 10.1007/5584_2020_588

Review

Exosomes as Part of the Human Adipose-Derived Stem Cells Secretome- Opening New Perspectives for Cell-Free Regenerative Applications

Sorina Dinescu et al. Adv Exp Med Biol. 2021.

. 2021:1312:139-163.

doi: 10.1007/5584_2020_588.

Authors

Sorina Dinescu^{1

2}, Alexandra Dobranici^#³, Ramona Tecucianu^#³, Aida Selaru^{3

4}, Roxana Balahura^{3

4}, Simona Ignat³, Marieta Costache^{3

5}

Affiliations

¹ Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania. sorina.dinescu@bio.unibuc.ro.
² The Research Institute of the University of Bucharest, Bucharest, Romania. sorina.dinescu@bio.unibuc.ro.
³ Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania.
⁴ Victor Babes National Institute of Pathology, Bucharest, Romania.
⁵ The Research Institute of the University of Bucharest, Bucharest, Romania.

^# Contributed equally.

PMID: 32986128
DOI: 10.1007/5584_2020_588

Abstract

Human adipose-derived stem cells (hASCs) represent a great resource for regenerative medicine based on their accessibility, self-renewal potential, low immunogenicity, high proliferative rate and potential to differentiate on multiple lineages. Their secretome is rich in chemokines, cytokines and protein growth factors that are actively involved in regeneration processes. In addition, part of this secretome are also the exosomes (hASC-exos), which display high content in proteins, messenger RNAs (mRNAs) and non-coding RNAs (ncRNAs). Due to their content, exosomes promote tissue regeneration by different mechanisms, either by activating or inhibiting several signaling pathways involved in wound healing, extracellular matrix remodeling, immunomodulation, angiogenesis, anti-apoptotic activity and cell migration, proliferation and differentiation. The use of hASC-exos may provide an improved alternative to standard therapies used in regenerative medicine, as a cell-free new approach with multiple possibilities to be modulated according to the patient needs. This review offers an updated overview on the functions and applications of hASC-exos in all areas of tissue regeneration, aiming to highlight to the reader the benefits of using hASCs in modern tissue engineering and regenerative medicine applications.

Keywords: Exosomes; Human adipose-derived stem cells; Signaling pathways in regeneration; Tissue regeneration; miRNAs.

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References

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1. An Y, Zhao J, Nie F et al (2019) Exosomes from adipose-derived stem cells (ADSCs) overexpressing miR-21 promote vascularization of endothelial cells. Sci Rep 9:12861. https://doi.org/10.1038/s41598-019-49339-y - DOI - PubMed - PMC
1. Anand S, Samuel M, Kumar S, Mathivanan S (2019) Ticket to a bubble ride: cargo sorting into exosomes and extracellular vesicles. Biochim Biophys Acta Proteins Proteomics 1867:140203. https://doi.org/10.1016/j.bbapap.2019.02.005 - DOI - PubMed
1. Bae S, Brumbaugh J, Bonavida B (2018) Exosomes derived from cancerous and non-cancerous cells regulate the anti-tumor response in the tumor microenvironment. Genes Cancer 9:87–100. https://doi.org/10.18632/genesandcancer.172 - DOI - PubMed - PMC
1. Bai Y, Han Y, Yan X et al (2018) Adipose mesenchymal stem cell-derived exosomes stimulated by hydrogen peroxide enhanced skin flap recovery in ischemia-reperfusion injury. Biochem Biophys Res Commun 500:310–317. https://doi.org/10.1016/j.bbrc.2018.04.065 - DOI - PubMed

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[1] Al-Ghadban S, Bunnell BA (2020) Adipose tissue-derived stem cells: immunomodulatory effects and therapeutic potential. Physiology 35:125–133. https://doi.org/10.1152/physiol.00021.2019 - DOI - PubMed

[2] Al-Ghadban S, Bunnell BA (2020) Adipose tissue-derived stem cells: immunomodulatory effects and therapeutic potential. Physiology 35:125–133. https://doi.org/10.1152/physiol.00021.2019 - DOI - PubMed

[3] An Y, Zhao J, Nie F et al (2019) Exosomes from adipose-derived stem cells (ADSCs) overexpressing miR-21 promote vascularization of endothelial cells. Sci Rep 9:12861. https://doi.org/10.1038/s41598-019-49339-y - DOI - PubMed - PMC

[4] An Y, Zhao J, Nie F et al (2019) Exosomes from adipose-derived stem cells (ADSCs) overexpressing miR-21 promote vascularization of endothelial cells. Sci Rep 9:12861. https://doi.org/10.1038/s41598-019-49339-y - DOI - PubMed - PMC

[5] Anand S, Samuel M, Kumar S, Mathivanan S (2019) Ticket to a bubble ride: cargo sorting into exosomes and extracellular vesicles. Biochim Biophys Acta Proteins Proteomics 1867:140203. https://doi.org/10.1016/j.bbapap.2019.02.005 - DOI - PubMed

[6] Anand S, Samuel M, Kumar S, Mathivanan S (2019) Ticket to a bubble ride: cargo sorting into exosomes and extracellular vesicles. Biochim Biophys Acta Proteins Proteomics 1867:140203. https://doi.org/10.1016/j.bbapap.2019.02.005 - DOI - PubMed

[7] Bae S, Brumbaugh J, Bonavida B (2018) Exosomes derived from cancerous and non-cancerous cells regulate the anti-tumor response in the tumor microenvironment. Genes Cancer 9:87–100. https://doi.org/10.18632/genesandcancer.172 - DOI - PubMed - PMC

[8] Bae S, Brumbaugh J, Bonavida B (2018) Exosomes derived from cancerous and non-cancerous cells regulate the anti-tumor response in the tumor microenvironment. Genes Cancer 9:87–100. https://doi.org/10.18632/genesandcancer.172 - DOI - PubMed - PMC

[9] Bai Y, Han Y, Yan X et al (2018) Adipose mesenchymal stem cell-derived exosomes stimulated by hydrogen peroxide enhanced skin flap recovery in ischemia-reperfusion injury. Biochem Biophys Res Commun 500:310–317. https://doi.org/10.1016/j.bbrc.2018.04.065 - DOI - PubMed

[10] Bai Y, Han Y, Yan X et al (2018) Adipose mesenchymal stem cell-derived exosomes stimulated by hydrogen peroxide enhanced skin flap recovery in ischemia-reperfusion injury. Biochem Biophys Res Commun 500:310–317. https://doi.org/10.1016/j.bbrc.2018.04.065 - DOI - PubMed

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Exosomes as Part of the Human Adipose-Derived Stem Cells Secretome- Opening New Perspectives for Cell-Free Regenerative Applications

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Exosomes as Part of the Human Adipose-Derived Stem Cells Secretome- Opening New Perspectives for Cell-Free Regenerative Applications

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