Adsorption protein of bacteriophage fl: solubilization in deoxycholate and localization in the fl virion
- PMID: 329864
- DOI: 10.1021/bi00631a017
Adsorption protein of bacteriophage fl: solubilization in deoxycholate and localization in the fl virion
Abstract
A complex containing the minor coat protein or adsorptionprotein (A protein) of bacteriophage fl has been solubilized from the fl virion, using the detergent deoxycholate. This complex was resolved from the fl DNA and from the fl major coat protein, or B protein, by gel filtration in the presence of deoxycholate. The A protein complex migrated as a single band on sodium dodecyl sulfate-urea-polyacrylamide gels corresponding to a molecular weight of 60 000. Analysis of the amino acid composition and amino terminal residues of this preparation indicates that the preparation contains a 20% contamination of additional protein species. Antibody against purified fd A protein is cross-reactive with deoxycholate-purified fl A protein and with fl phage. Electron microscopic observation of negatively stained complexes of fl phage with this anti-fd A protein antibody and ferritin conjugated goat anti-rabbit IgG antibody revealed phages with ferritin particles at their termini or complexes of two or more phages joined together at one end by ferritin, indicating that the complex of A protein molecules is located at one end of the filamentous fl virion.
Similar articles
-
Adsorption protein of the bacteriophage fd: isolation, molecular properties, and location in the virus.Biochemistry. 1977 Jun 14;16(12):2686-94. doi: 10.1021/bi00631a016. Biochemistry. 1977. PMID: 329863
-
Structure of the filamentous bacteriophage fl. Location of the A, C, and D minor coat proteins.J Biol Chem. 1981 Jan 10;256(1):539-46. J Biol Chem. 1981. PMID: 7005219
-
Interaction of deoxycholate and of detergents with the coat protein of bacteriophage f1.J Biol Chem. 1975 Jun 10;250(11):4327-32. J Biol Chem. 1975. PMID: 1092689
-
Structure of filamentous bacteriophage: isolation, characterization, and localization of the minor coat proteins and orientation of the DNA.Prog Clin Biol Res. 1981;64:413-28. Prog Clin Biol Res. 1981. PMID: 7330056
-
Proteolytic digestion of the micellar complex of f1 coat protein and deoxycholate.J Biol Chem. 1975 Jun 10;250(11):4333-9. J Biol Chem. 1975. PMID: 1126954
Cited by
-
A hybrid toxin from bacteriophage f1 attachment protein and colicin E3 has altered cell receptor specificity.J Bacteriol. 1988 Sep;170(9):4231-8. doi: 10.1128/jb.170.9.4231-4238.1988. J Bacteriol. 1988. PMID: 3045089 Free PMC article.
-
fii, a bacterial locus required for filamentous phage infection and its relation to colicin-tolerant tolA and tolB.J Bacteriol. 1986 Jan;165(1):107-15. doi: 10.1128/jb.165.1.107-115.1986. J Bacteriol. 1986. PMID: 3001021 Free PMC article.
-
Mechanisms of membrane assembly: effects of energy poisons on the conversion of soluble M13 coliphage procoat to membrane-bound coat protein.Proc Natl Acad Sci U S A. 1980 Feb;77(2):827-31. doi: 10.1073/pnas.77.2.827. Proc Natl Acad Sci U S A. 1980. PMID: 6928682 Free PMC article.
-
The major coat protein gene of the filamentous Pseudomonas aeruginosa phage Pf3: absence of an N-terminal leader signal sequence.Nucleic Acids Res. 1983 Nov 25;11(22):8073-85. doi: 10.1093/nar/11.22.8073. Nucleic Acids Res. 1983. PMID: 6316283 Free PMC article.
-
Tethering of Epidermal Growth Factor (EGF) to Beta Tricalcium Phosphate (βTCP) via Fusion to a High Affinity, Multimeric βTCP-Binding Peptide: Effects on Human Multipotent Stromal Cells/Connective Tissue Progenitors.PLoS One. 2015 Jun 29;10(6):e0129600. doi: 10.1371/journal.pone.0129600. eCollection 2015. PLoS One. 2015. PMID: 26121597 Free PMC article.