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. 1977 Sep 6;478(1):109-13.
doi: 10.1016/0005-2787(77)90249-0.

Unidirectional replication of the P-group plasmid RK2

R J Meyer, D R Helinski

Unidirectional replication of the P-group plasmid RK2

R J Meyer et al. Biochim Biophys Acta. .

Abstract

The mode of replication of the broad host-range plasmid RK2 has been determined from examination of molecular replicative forms cleaved with the restriction endonucleases EcoRI and Hind III. Replication is unidirectional, and proceeds from a unique origin. The location of the origin and other evidence suggests that genes involved in plasmid maintenance are not tightly clustered.

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