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. 2020 Dec;5(12):1459-1463.
doi: 10.1038/s41564-020-00793-9. Epub 2020 Sep 28.

Escherichia coli is engineered to grow on CO2 and formic acid

Junho Bang  1   2 , Chang Hun Hwang  1   2 , Jung Ho Ahn  1   2 , Jong An Lee  1   2 , Sang Yup Lee  3   4   5
Affiliations

Escherichia coli is engineered to grow on CO2 and formic acid

Junho Bang et al. Nat Microbiol. 2020 Dec.

Abstract

We engineered Escherichia coli to grow on CO2 and formic acid alone by introducing the synthetic CO2 and formic acid assimilation pathway, expressing two formate dehydrogenase genes, fine-tuning metabolic fluxes and optimizing the levels of cytochrome bo3 and bd-I ubiquinol oxidase. Our engineered strain can grow to an optical density at 600 nm of 7.38 in 450 h, and shows promise as a platform strain growing on CO2 and formic acid alone.

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