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. 1987 Jun 25;15(12):4761-70.
doi: 10.1093/nar/15.12.4761.

Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes

J A Garson, J A van den Berghe, J T Kemshead
Free PMC article

Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes

J A Garson et al. Nucleic Acids Res. .
Free PMC article

Abstract

A novel in situ hybridization technique is described. This non-radioactive technique combines, for the first time, the high spacial resolution and rapid signal development of the non-isotopic approach with the previously unrivalled sensitivity of autoradiography. The procedure, which employs biotin labelled DNA probes and a streptavidin-alkaline phosphatase based detection system, is compatible with pre G-banding and can be performed on archival material. Unique sequences as small as 1 Kb are detectable. Using this technique, we have mapped the N-myc oncogene and the gene for beta-Nerve Growth Factor to 2p24 and 1p13 respectively.

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References

    1. Cytogenet Cell Genet. 1985;39(3):184-7 - PubMed
    1. Nature. 1985 Sep 12-18;317(6033):175-7 - PubMed
    1. Histochemistry. 1986;84(2):179-85 - PubMed
    1. Cytogenet Cell Genet. 1986;42(1-2):105-9 - PubMed
    1. Hum Genet. 1986 Aug;73(4):354-7 - PubMed

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