Sea Buckthorn and Rosehip Oils with Chokeberry Extract to Prevent Hypercholesterolemia in Mice Caused by a High-Fat Diet In Vivo

Abstract

Dietary supplementation based on sea buckthorn and rosehip oils with added chokeberry extract was studied. We added the dietary supplement to the feed mixtures for laboratory animals. The possible toxicological effects and hypocholesterolemic, hepatoprotective activity of the dietary supplement in vivo were studied. After the observation period (6 weeks), no significant changes were found in the mass of organs and blood serum of laboratory animals (p > 0.05). However, there was a decrease in hypercholesterolemic indicators. Regular consumption of sea buckthorn and rosehip oils with added chokeberry extract (dietary supplement "ESB-1") by laboratory animals inhibited the activity of liver enzymes and increased the antioxidant activity of blood serum (after the subcutaneous injection of sunflower oil/oil solution of carbon tetrachloride) but was not sufficient to bring them to physiological standards. The hypocholesterolemic and antioxidant properties of our dietary supplement already allow us to consider it a component of functional food products or a dietary supplement base. However, the full range of its biologically active properties, including the hepatoprotective function and regulation of metabolic disorders, has not been studied yet, which sets the direction of further research in vivo models and clinical practice to confirm its effectiveness in humans.

Keywords: antioxidant activity; chokeberry; dietary supplement; hepatoprotective effects; hypolipidemic; sea buckthorn and rosehip oils.

Figure 1

Biochemical and hematological indices of blood of animals from groups I–VII: 1—Cholesterol, mmol/L; 2—Triglycerides, mmol/L; 3—Bilirubin, μmol/L; 4—Number of leukocytes, g/L; 5—Hematocrit, L/L (bars in graphs indicate the SE of 15 replicates). Values with «*» do differ significantly (p > 0.05).

Figure 2

Biochemical and hematological indices of blood of animals from groups I–VII: 6—Urea, mmol/L; 7—ALT, U/L; 8—AST, U/L; 9—Globulins, g/L; 10—Red blood cell count, g/L; (bars in graphs indicate the SE of 15 replicates).

Figure 3

Biochemical and hematological indices of blood of animals from groups I–VII: 11—Total protein, g/L; 12—Albumin, g/L; 13—Creatinine, μmol/L; 14—Hemoglobin level, g/L; 15—Platelet count, g/L; 16—Average concentration hemoglobin in the erythrocyte, g/L (bars in graphs indicate the SE of 15 replicates).