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. 2020 Sep 29;10(1):16053.
doi: 10.1038/s41598-020-72679-z.

Edaphic factors and plants influence denitrification in soils from a long-term arable experiment

Ian M Clark  1 , Qingling Fu  1   2 , Maïder Abadie  1 , Elizabeth R Dixon  3 , Aimeric Blaud  1   4 , Penny R Hirsch  5
Affiliations

Edaphic factors and plants influence denitrification in soils from a long-term arable experiment

Ian M Clark et al. Sci Rep. .

Abstract

Factors influencing production of greenhouse gases nitrous oxide (N2O) and nitrogen (N2) in arable soils include high nitrate, moisture and plants; we investigate how differences in the soil microbiome due to antecedent soil treatment additionally influence denitrification. Microbial communities, denitrification gene abundance and gas production in soils from tilled arable plots with contrasting fertilizer inputs (no N, mineral N, FYM) and regenerated woodland in the long-term Broadbalk field experiment were investigated. Soil was transferred to pots, kept bare or planted with wheat and after 6 weeks, transferred to sealed chambers with or without K15NO3 fertilizer for 4 days; N2O and N2 were measured daily. Concentrations of N2O were higher when fertilizer was added, lower in the presence of plants, whilst N2 increased over time and with plants. Prior soil treatment but not exposure to N-fertiliser or plants during the experiment influenced denitrification gene (nirK, nirS, nosZI, nosZII) relative abundance. Under our experimental conditions, denitrification generated mostly N2; N2O was around 2% of total gaseous N2 + N2O. Prior long-term soil management influenced the soil microbiome and abundance of denitrification genes. The production of N2O was driven by nitrate availability and N2 generation increased in the presence of plants.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Relative mean abundance of prokaryotic phyla/sub-phyla in soils of origin on collection from the field. Phyla with at least 0.1% of the total community present in at least one soil treatment are included. Proteobacteria sub-phyla: a = alpha, b = beta, d = delta, g = gamma; s.e.d. for each group is shown; letters indicate mean significantly different means within each group (P = 0.05, according to Tukey’s post-hoc test on ANOVA). Insert top right shows NMDS plot of OTU for prokaryotic communities – PERMANOVA F = 9.477, P (same) = 0.0001.
Figure 2
Figure 2
Gene abundance from qPCR at the end of the experiment, pooling all treatments for each soil of origin (n = 12); letters denote significantly different values within each set of genes (P = 0.05) according to Tukey’s post-hoc test in ANOVA; s.e.d. = standard errors of difference of means; note that 16S and nirK are plotted as 10–9, the other genes as 10–6 copies g−1 dw soil.
Figure 3
Figure 3
Soil properties at the end of the experiment. (a) concentration of NH4+-N; (b) NO3–-N; (c) % wfps; means for soils with all treatments (n = 3); different letters denote significantly different values according to Tukey’s post-hoc test in ANOVA (P = 0.05), s.e.d. = standard errors of difference of means for all samples.
Figure 4
Figure 4
Mean gas production over 4 days. (a) N2O-N, all treatments (48 pots); (b–e) K15NO3-fertilized treatments only (24 pots). (b) 15N atom % measured in N2O; (c) N2O-N indicating %15N (upper s.e.d. relates to N2O-N; lower bar relates to 15N atom %), (d) N2-N, (e) N2O-N as % (N2-N + N2O-N). Different letters denote significantly different values according to Tukey’s post-hoc test in ANOVA (P = 0.05), s.e.d. = standard errors of difference of means for all samples.
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References

    1. Smith KA. Changing views of nitrous oxide emissions from agricultural soil: Key controlling processes and assessment at different spatial scales. Eur. J. Soil Sci. 2017;68:137–155. doi: 10.1111/ejss.12409. - DOI
    1. Philippot L, Hallin S, Schloter M. Ecology of denitrifying prokaryotes in agricultural soil. Adv. Agron. 2007;96(96):249–305. doi: 10.1016/S0065-2113(07)96003-4. - DOI
    1. Thomson AJ, Giannopoulos G, Pretty J, Baggs EM, Richardson DJ. Biological sources and sinks of nitrous oxide and strategies to mitigate emissions. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 2012;367:1157–1168. doi: 10.1098/rstb.2011.0415. - DOI - PMC - PubMed
    1. Bouwman AF. Direct emission of nitrous oxide from agricultural soils. Nutr. Cycl. Agroecosyst. 1996;46:53–70. doi: 10.1007/Bf00210224. - DOI
    1. Jones CM, Graf DRH, Bru D, Philippot L, Hallin S. The unaccounted yet abundant nitrous oxide-reducing microbial community: A potential nitrous oxide sink. ISME J. 2013;7:417–426. doi: 10.1038/ismej.2012.125. - DOI - PMC - PubMed

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