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. 2020 Mar 1;10(1):1-8.
doi: 10.6705/j.jacme.202003_10(1).0001.

Association Between Acute Inflammatory Cells and Mutation in ICAM-1 Gene With Injury Severity and Outcome Among Traumatic Cerebral Hemorrhagic Contusion

Samah Abdelrahman Hassan  1   2 , Alsadig Gassoum  3 , Sawsan Ahmed Hamed Aldeaf  3 , Mohamed Abdel Rahman Arbab  3   4 , Hassan Hussein Musa  5   6
Affiliations

Association Between Acute Inflammatory Cells and Mutation in ICAM-1 Gene With Injury Severity and Outcome Among Traumatic Cerebral Hemorrhagic Contusion

Samah Abdelrahman Hassan et al. J Acute Med. .

Abstract

Background: Acute inflammation in the brain after trauma is mediated by acute inflammatory cells (neutrophils) that contributes to ischemic brain damage, neurological deterioration, and poor outcome. Migration of neutrophils to brain is mediated by intercellular adhesion molecule-1 (ICAM-1). The aim was to determine an association between neutrophils counts, ICAM-1 level and mutation in ICAM-1 gene with injury severity and patient's outcome.

Methods: Cross-sectional study was conducted for ninety Sudanese patients presented with traumatic cerebral hemorrhagic contusion to the National Center for Neurological Sciences, Khartoum, Sudan from December 2015 to January 2018. Non-Sudanese patients, and hemorrhagic contusion associated with other type of brain bleeding were excluded in this study. Moreover, 90 apparently healthy individuals were participated as control.

Results: Most patients were males (93.3%), their ages ranged from 25 to 44 years, 11.1% of the patients had severe brain injury, 22.2% had brain edema and the mortality rate was 8.9%. Circulatory levels of leukocytes, neutrophil and ICAM-1 among patients who sustained trauma were significantly elevated compared with controls (p = 0.000). The high level of leukocytes and neutrophils counts were significantly associated with ICAM-1pg/mL circulatory level. High levels of leukocytes, neutrophils, and ICAM-1 were documented in severe brain injuries, whereas. high level of ICAM-1 was observed among patients admitted with brain edema. Leukocytes and neutrophils counts were significantly associated with patient outcome. High level of ICAM-1 (304.88 pg/mL) was observed among patients with poor outcome compared to survivals (263.93 pg/mL). The highest circulatory level of ICAM-1 (280.75 pg/mL) was observed among patient having adenine-adenine (AA) mutant homozygous alleles, followed by (272 pg/mL) for guanine-guanine (GG) homozygous alleles, then (245.12 pg/mL) for guanine-adenine (GA) heterozygous alleles.

Conclusions: Mutation in ICAM-1 gene and increased levels of leukocytes, neutrophils and ICAM-1 constitutes important markers for injury severity and patient's outcome.

Keywords: Cerebral contusion; ICAM-1; Inflammation; Neutrophils.

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Conflict of interest statement

Non-declared

Figures

Figure 1
Figure 1. Agarose gel electrophoresis of PCR products of intercellular adhesion molecule-1 (ICAM-1) gene, digested with BstUI restriction enzyme. Lane 1: deoxyribonucleic acid ladder. Land 2, 3, 5, 6, and 7: guanine-guanine (GG) homozygous alleles (band at 223 bp). Land 8: guanine-adenine (GA) heterozygous alleles (band at 223, 136, and 87 bp). And Land 4: adenine-adenine (AA) homozygous alleles (band at 136 and 87 bp).
Figure 2
Figure 2. Association between intercellular adhesion molecule-1 (ICAM-1) gene genotypes with ICAM-1 circulatory levels (p -value of ANOVA test = 0.757).
See this image and copyright information in PMC

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