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. 2020 Aug 27:13:119-129.
doi: 10.1016/j.ijppaw.2020.08.003. eCollection 2020 Dec.

Boehmiella wilsoni (Nematoda, Heligmosomoidea, Boehmiellidae fam. nov.), found in Amazonian rodents

B E Andrade-Silva  1   2 , R V Vilela  2 , E J Lopes-Torres  3 , S F Costa-Neto  4 , A Maldonado Jr  2
Affiliations

Boehmiella wilsoni (Nematoda, Heligmosomoidea, Boehmiellidae fam. nov.), found in Amazonian rodents

B E Andrade-Silva et al. Int J Parasitol Parasites Wildl. .

Abstract

The genus Boehmiella was initially described as a member of the family Trichostrongylidae. Subsequently, it was assigned to the subfamily Haemonchinae in the family Haemonchidae. We analyzed parasites of spiny tree-rats, Mesomys hispidus, collected in the Amazon rainforest, which were identified as B. wilsoni based on integrative taxonomy. Using morphology, morphometry, and scanning electron microscopy (SEM), we added new data to the original description of the species. We also inferred phylogenetic hypotheses for its relationships within the Trichostrongylina, based on partial nuclear 18S and 28S rRNA genes, through Maximum Likelihood and Bayesian analyses. In conclusion, B. wilsoni does not belong to the family Haemonchidae, nor is it closely related to any other trichostrongylin family, and therefore, we propose the establishment of a new family, Boehmiellidae fam. nov., to which the genus Boehmiella is allocated.

Keywords: 18S rRNA; 28; Echimyidae; Molecular phylogenetics; Scanning electron microscopy.

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Conflict of interest statement

The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Figures

Image 1
Graphical abstract
Fig. 1
Fig. 1
Light microscopy with camera lucida of Boehmiella wilsoni. (A) Anterior part of female body. (B) Neodont. (C) Cross-section of the head, with the neodont and denticles in detail. (D) Posterior part of female body. (E) Dissected ovejector. (F) Dorsal rays. (G) Posterior part of male body. (H) Telamon. (I) Gubernaculum. (J) Posterior part of male body, copulatory bursal closed.
Fig. 2
Fig. 2
Light microscopy of Boehmiella wilsoni. (A) Posterior part of male body, gubernaculum (asterisk). (B) Spicule. (C) Posterior part of female body, vulva (arrow). (D) Dissected ovejector. (E) Uterus with eggs. (F) Spermatheca.
Fig. 3
Fig. 3
Light microscopy with camera lucida of Boehmiella wilsoni. (A) Cross-section of the body in the cervical region. (B) Cross-section of the body in the middle region. (C) Cross-section of the body in the posterior extremity (Female). (D) Cross-section of the body in the cervical region. (E) Cross-section of the body in the middle region. (F) Cross-section of the body in the posterior extremity (Male).
Fig. 4
Fig. 4
Scanning electron microscopy of Boehmiella wilsoni. (A) Anterior region showing a deirid (arrowhead) and the excretory pore (arrow). (B) Detail of a deirid. (C) Detail of excretory pore. (D) Anterior end in apical view showing two cephalic papillae (pc) and amphid (a). (E) Posterior end of female and detail of anus (arrow). (F) Posterior end of male, showing the prebursal papillae (arrow) and spicule tip(s). (G) Detail of a prebursal papillae (p). (H) Detail of a pair of spicule tips. Abbreviation: v-ventral and d-dorsal.
Fig. 5
Fig. 5
18S gene matrix strict consensus cladogram of ML and BI analyses.
Fig. 6
Fig. 6
28S gene matrix strict consensus cladogram of ML and BI analyses.
Fig. 7
Fig. 7
Phylogenetic relationships of Boehmiella wilsoni, Trichostrongylina, and outgroup sequences. Concatenated 18S and 28S genes matrix ML phylogram. Support values at nodes: aLRT/ML-BP/BPP, respectively.
See this image and copyright information in PMC

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