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. 2020 Sep 23;35(9):e202000901.
doi: 10.1590/s0102-865020200090000001. eCollection 2020.

Evaluation of Indigo carmine on hepatic ischemia and reperfusion injury

Eduardo Alexandre Rancan  1 , Eloísa Ianes Frota  1 , Tábata Marina Nóbrega de Freitas  1 , Maria Cecília Jordani  2 , Paulo Roberto Barbosa Évora  3 , Orlando Castro-E-Silva  4
Affiliations

Evaluation of Indigo carmine on hepatic ischemia and reperfusion injury

Eduardo Alexandre Rancan et al. Acta Cir Bras. .

Abstract

Purpose: To evaluate the effects of treatment with Indigo Carmine (IC) on rat livers subjected to ischemia-reperfusion injury.

Methods: The animals were subdivided into 4 groups: 1.SHAM group(SH) - saline; 2.SHAM group with IC-2mg/Kg(SHIC); 3.IR group - rats submitted to ischemia and reperfusion with saline(IR); 4.IR group with IC-2mg/Kg(IRIC). The IR protocol consists of liver exposure and administration of drug or saline intravenously, followed by 60 minutes of ischemia and 15 of reperfusion. Liver samples were collected for biochemical analysis.

Results: State 3 of mitochondrial respiration showed a significant worsening of the IRIC group in relation to all others. State 4 showed a difference between IRIC and SHIC. The Respiratory Control Ratio showed statistical decrease in IR and IRIC versus Sham. The osmotic swelling showed significant difference between SHxIR; SHICxIRIC and SHxIRIC. There was a significant increase in ALT in the IRIC group in relation to all the others. Concerning the nitrate dosage, there was a decrease in the group treated with IC(IRxIRIC). There was no difference regarding the dosage of Malondialdehyde.

Conclusion: IC was not able to protect mitochondria from IR injury and proved to be a potentiating agent, acting in synergy with the IR injury promoting damage to the hepatocyte membranes.

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Conflict of interest statement

Conflict of interest: none

Figures

Figure 1
Figure 1. (A) The oxygen consumption by mitochondria in the presence of ADP – State 3, values expressed in nmol / min / mg (p<0.05: SH x IR; SHIC x IRIC; SH x IRIC; IR x IRIC). (B) The baseline oxygen consumption by mitochondria – State 4, values expressed in nmol / min / mg (p<0.05: SHIC x IRIC). (C) The State 3/ State 4 ratio, respiratory control ratio (RCR) (p<0.05: SH x IR; SH x IRIC). (D) Mitochondrial swelling induced by CaCl2 and KH2 PO4. p <0.05: SH x IR; SHIC x IRIC; SH x IRIC.
Figure 2
Figure 2. (A) MDA, there was no statistically significant difference between groups. (B) NO, p<0.05: SHIC x IR; IR x IRIC.
Figure 3
Figure 3. (A) ALT, p<0.05: SH x IR; SHIC x IRIC; SH x IRIC; IR x IRIC. (B) AST, p<0.05: SH x IR; SHIC x IRIC; SH x IRIC. (C) LDH, p <0.05: SH x IR; SH x IRIC; SHIC x IR.
See this image and copyright information in PMC

References

    1. Castro e Silva O, Jr, Centurion S, Pacheco E, Brisotti J, Oliveira A, Dal Sasso K. Basics aspects of the ischemia reperfusion injury and of the ischemic preconditioning. Acta Cir Bras. 2002;17(3):96–100. doi: 10.1590/S0102-86502002000900020. - DOI
    1. Van Riel WG, Van Golen RF, Reiniers MJ, Heger M, Van Gulik TM. How much ischemia can the liver tolerate during resection? Hepatobiliary Surg Nutr. 2016;5(1):58–71. doi: 10.3978/j.issn.2304-3881.2015.07.05. - DOI - PMC - PubMed
    1. Teixeira AC, Souza FF, GdA Mota, AdLC Martinelli, Sankarankutty AK, Castro e Silva O. Liver transplantation: expectation with MELD score for liver allocation in Brazil. 10.1590/S0102-86502006000700003Acta Cir Bras. 2006;21:12–14. - PubMed
    1. Peralta C, Jiménez-Castro MB, Gracia-Sancho J. Hepatic ischemia and reperfusion injury: effects on the liver sinusoidal milieu. 10.1016/j.jhep.2013.06.017J. 2013;59(5):1094–1106. - PubMed
    1. Cannistrà M, Ruggiero M, Zullo A, Gallelli G, Serafini S, Maria M, Naso A, Grande R, Serra R, Nardo B. Hepatic ischemia reperfusion injury: A systematic review of literature and the role of current drugs and biomarkers. 10.1016/j.ijsu.2016.05.050Int J Surg. 2016;33:S57–S70. - PubMed