Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2020 Nov;32(6):964-967.
doi: 10.1177/1040638720963132. Epub 2020 Oct 1.

CD3-CD20-positive nodal lymphoma with cross-lineage rearrangement in a dog

Arturo Nicoletti  1 , Luca Aresu  1 , Michele Marino  2 , Maria Massaro  2 , Eugenio Martignani  1 , Elisa Caporali  3 , Silvia Capuccini  3 , Ugo Bonfanti  2 , Cecilia Gola  1
Affiliations
Case Reports

CD3-CD20-positive nodal lymphoma with cross-lineage rearrangement in a dog

Arturo Nicoletti et al. J Vet Diagn Invest. 2020 Nov.

Abstract

A 7-y-old mixed-breed male dog was presented with a history of generalized lymphadenopathy. Fine-needle aspirates of the enlarged peripheral lymph nodes were suggestive of lymphoma. Histologic examination of a retromandibular lymph node was suggestive of high-grade, medium large-cell lymphoma. Immunohistochemistry revealed concurrent expression of CD3 and CD20. The co-localization of the 2 antigens was confirmed by immunofluorescence. PCR for antigen receptor gene rearrangements (PARR) detected clonal rearrangements for both T-cell receptor gamma and B-cell receptor. The final diagnosis was CD3-CD20-positive anaplastic lymphoma with cross-lineage rearrangement.

Keywords: CD3/CD20 positive; PARR; canine nodal lymphoma; dogs.

PubMed Disclaimer

Conflict of interest statement

Declaration of conflicting interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

Figures

Figure 1.
Figure 1.
Nodal lymphoma in a dog. A. Sheets of densely packed neoplastic cells infiltrating the lymph node compress the peripheral sinus and infiltrate the perinodal tissue. H&E. 100×. B. Round, intermediate-to-large, neoplastic lymphoid cells with distinct cell borders with marked anisocytosis and anisokaryosis, cleaved or oval-shaped nuclei, and granular chromatin. H&E. 600×. C. Diffuse and strong labeling of neoplastic lymphocytes with anti-CD3 antibody. IHC. 100×. Inset: cytoplasmic localization. IHC. 600×. D. Diffuse and strong labeling of neoplastic lymphocytes with anti-CD20 antibody. IHC. 100×. Inset: membranous localization. IHC. 600×.
Figure 2.
Figure 2.
Nuclear counterstain by DAPI (A), and labeling of neoplastic lymphocytes in the lymph node with anti-CD20 (B), and anti-CD3 (C) shows membranous and cytoplasmic co-localization (merged in D). Double immunofluorescent staining. 600×.
See this image and copyright information in PMC

References

    1. Aresu L, et al. Canine indolent and aggressive lymphoma: clinical spectrum with histologic correlation. Vet Comp Oncol 2015;13:348–362. - PubMed
    1. Bagg A. Immunoglobulin and T-cell receptor gene rearrangements: minding your B’s and T’s in assessing lineage and clonality in neoplastic lymphoproliferative disorders. J Mol Diagn 2006;8:426–429. - PMC - PubMed
    1. Bennett AL, et al. Canine acute leukaemia: 50 cases (1989–2014). Vet Comp Oncol 2017;15:1101–1114. - PMC - PubMed
    1. Brachelente C, et al. CD3 and CD20 coexpression in a case of canine cutaneous epitheliotropic T-cell lymphoma (mycosis fungoides). Vet Pathol 2016;53:563–566. - PubMed
    1. Cortelazzo S, et al. Lymphoblastic lymphoma. Crit Rev Oncol Hematol 2011;79:330–343. - PubMed

Publication types

MeSH terms

LinkOut - more resources