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. 2020 Dec 1;59(49):21858-21863.
doi: 10.1002/anie.202009544. Epub 2020 Sep 30.

Dye-Doped Silica Nanoparticles for Enhanced ECL-Based Immunoassay Analytical Performance

Alessandra Zanut  1   2 , Francesco Palomba  1   3 , Matilde Rossi Scota  1 , Sara Rebeccani  1 , Massimo Marcaccio  1 , Damiano Genovese  1 , Enrico Rampazzo  1 , Giovanni Valenti  1 , Francesco Paolucci  1 , Luca Prodi  1
Affiliations

Dye-Doped Silica Nanoparticles for Enhanced ECL-Based Immunoassay Analytical Performance

Alessandra Zanut et al. Angew Chem Int Ed Engl. .

Abstract

The combination of highly sensitive techniques such as electrochemiluminescence (ECL) with nanotechnology sparked new analytical applications, in particular for immunoassay-based detection systems. In this context, nanomaterials, particularly dye-doped silica nanoparticles (DDSNPs) are of high interest, since they can offer several advantages in terms of sensitivity and performance. In this work we synthesized two sets of monodispersed and biotinylated [Ru(bpy)3 ]2+ -doped silica nanoparticles, named bio-Triton@RuNP and bio-Igepal@RuNP, obtained following the reverse microemulsion method using two different types of nonionic surfactants. Controlling the synthetic procedures, we were able to obtain nanoparticles (NPs) offering highly intense signal, using tri-n-propylamine (TPrA) as coreactant, with bio-Triton@RuNps being more efficient than bio-Igepal@RuNP.

Keywords: ECL imaging; electrochemiluminescence; electrochemistry; nanomaterials; silica nanoparticles.

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References

    1. N. Sojic, Analytical Electrogenerated Chemiluminescence: From Fundamentals to Bioassays. Detection Science, Royal Society Of Chemistry (RSC) Publishing, 2020.
    1. M. Hesari, Z. Ding, J. Electrochem. Soc. 2016, 163, H3116-H3131.
    1. M. M. Richter, Chem. Rev. 2004, 104, 3003-3036.
    1. A. Zanut, A. Fiorani, S. Canola, T. Saito, N. Ziebart, S. Rapino, S. Rebeccani, A. Barbon, T. Irie, H.-P. Josel, et al., Nat. Commun. 2020, https://doi.org/10.1038/s41467-020-16476-2.
    1. Y. Cao, X. Gou, J. Zhu, Anal. Chem. 2020, 92, 431-454.

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