The science and engineering of stem cell-derived organoids-examples from hepatic, biliary, and pancreatic tissues

doi:10.1111/brv.12650

Review

. 2021 Feb;96(1):179-204.

doi: 10.1111/brv.12650. Epub 2020 Oct 1.

The science and engineering of stem cell-derived organoids-examples from hepatic, biliary, and pancreatic tissues

Ogechi Ogoke¹, Mitchell Maloy¹, Natesh Parashurama^{1

2

3}

Affiliations

¹ Department of Chemical and Biological Engineering, University at Buffalo (State University of New York), Buffalo, NY, U.S.A.
² Clinical and Translation Research Center (CTRC), University at Buffalo (State University of New York), Buffalo, NY, U.S.A.
³ Department of Biomedical Engineering, University at Buffalo (State University of New York), Buffalo, NY, U.S.A.

PMID: 33002311
DOI: 10.1111/brv.12650

Review

The science and engineering of stem cell-derived organoids-examples from hepatic, biliary, and pancreatic tissues

Ogechi Ogoke et al. Biol Rev Camb Philos Soc. 2021 Feb.

. 2021 Feb;96(1):179-204.

doi: 10.1111/brv.12650. Epub 2020 Oct 1.

Authors

Ogechi Ogoke¹, Mitchell Maloy¹, Natesh Parashurama^{1

2

3}

Affiliations

¹ Department of Chemical and Biological Engineering, University at Buffalo (State University of New York), Buffalo, NY, U.S.A.
² Clinical and Translation Research Center (CTRC), University at Buffalo (State University of New York), Buffalo, NY, U.S.A.
³ Department of Biomedical Engineering, University at Buffalo (State University of New York), Buffalo, NY, U.S.A.

PMID: 33002311
DOI: 10.1111/brv.12650

Abstract

The field of organoid engineering promises to revolutionize medicine with wide-ranging applications of scientific, engineering, and clinical interest, including precision and personalized medicine, gene editing, drug development, disease modelling, cellular therapy, and human development. Organoids are a three-dimensional (3D) miniature representation of a target organ, are initiated with stem/progenitor cells, and are extremely promising tools with which to model organ function. The biological basis for organoids is that they foster stem cell self-renewal, differentiation, and self-organization, recapitulating 3D tissue structure or function better than two-dimensional (2D) systems. In this review, we first discuss the importance of epithelial organs and the general properties of epithelial cells to provide a context and rationale for organoids of the liver, pancreas, and gall bladder. Next, we develop a general framework to understand self-organization, tissue hierarchy, and organoid cultivation. For each of these areas, we provide a historical context, and review a wide range of both biological and mathematical perspectives that enhance understanding of organoids. Next, we review existing techniques and progress in hepatobiliary and pancreatic organoid engineering. To do this, we review organoids from primary tissues, cell lines, and stem cells, and introduce engineering studies when applicable. We discuss non-invasive assessment of organoids, which can reveal the underlying biological mechanisms and enable improved assays for growth, metabolism, and function. Applications of organoids in cell therapy are also discussed. Taken together, we establish a broad scientific foundation for organoids and provide an in-depth review of hepatic, biliary and pancreatic organoids.

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References

REFERENCES

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1. Asai, A., Aihara, E., Watson, C., Mourya, R., Mizuochi, T., Shivakumar, P., Phelan, K., Mayhew, C., Helmrath, M., Takebe, T., Wells, J. & Bezerra, J. A. (2017). Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells. Development 144(6), 1056-1064.
1. Augsornworawat, P., Velazco-Cruz, L., Song, J. & Millman, J. R. (2019). A hydrogel platform for in vitro three dimensional assembly of human stem cell-derived islet cells and endothelial cells. Acta Biomaterialia 97, 272-280.
1. Baccarani, U., Adani, G. L., Sainz, M., Donini, A., Risaliti, A. & Bresadola, F. (2005). Human hepatocyte transplantation for acute liver failure: state of the art and analysis of cell sources. Transplant Proceedings 37(6), 2702-2704.
1. Bhatia, S. N., Balis, U. J., Yarmush, M. L. & Toner, M. (1998). Microfabrication of hepatocyte/fibroblast co-cultures: role of homotypic cell interactions. Biotechnology Progress 14(3), 378-387.

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[1] Akbari, S., Sevinc, G. G., Ersoy, N., Basak, O., Kaplan, K., Sevinc, K., Ozel, E., Sengun, B., Enustun, E., Ozcimen, B., Bagriyanik, A., Arslan, N., Onder, T. T. & Erdal, E. (2019). Robust, long-term culture of endoderm-derived hepatic organoids for disease modeling. Stem Cell Reports 13(4), 627-641.

[2] Akbari, S., Sevinc, G. G., Ersoy, N., Basak, O., Kaplan, K., Sevinc, K., Ozel, E., Sengun, B., Enustun, E., Ozcimen, B., Bagriyanik, A., Arslan, N., Onder, T. T. & Erdal, E. (2019). Robust, long-term culture of endoderm-derived hepatic organoids for disease modeling. Stem Cell Reports 13(4), 627-641.

[3] Asai, A., Aihara, E., Watson, C., Mourya, R., Mizuochi, T., Shivakumar, P., Phelan, K., Mayhew, C., Helmrath, M., Takebe, T., Wells, J. & Bezerra, J. A. (2017). Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells. Development 144(6), 1056-1064.

[4] Asai, A., Aihara, E., Watson, C., Mourya, R., Mizuochi, T., Shivakumar, P., Phelan, K., Mayhew, C., Helmrath, M., Takebe, T., Wells, J. & Bezerra, J. A. (2017). Paracrine signals regulate human liver organoid maturation from induced pluripotent stem cells. Development 144(6), 1056-1064.

[5] Augsornworawat, P., Velazco-Cruz, L., Song, J. & Millman, J. R. (2019). A hydrogel platform for in vitro three dimensional assembly of human stem cell-derived islet cells and endothelial cells. Acta Biomaterialia 97, 272-280.

[6] Augsornworawat, P., Velazco-Cruz, L., Song, J. & Millman, J. R. (2019). A hydrogel platform for in vitro three dimensional assembly of human stem cell-derived islet cells and endothelial cells. Acta Biomaterialia 97, 272-280.

[7] Baccarani, U., Adani, G. L., Sainz, M., Donini, A., Risaliti, A. & Bresadola, F. (2005). Human hepatocyte transplantation for acute liver failure: state of the art and analysis of cell sources. Transplant Proceedings 37(6), 2702-2704.

[8] Baccarani, U., Adani, G. L., Sainz, M., Donini, A., Risaliti, A. & Bresadola, F. (2005). Human hepatocyte transplantation for acute liver failure: state of the art and analysis of cell sources. Transplant Proceedings 37(6), 2702-2704.

[9] Bhatia, S. N., Balis, U. J., Yarmush, M. L. & Toner, M. (1998). Microfabrication of hepatocyte/fibroblast co-cultures: role of homotypic cell interactions. Biotechnology Progress 14(3), 378-387.

[10] Bhatia, S. N., Balis, U. J., Yarmush, M. L. & Toner, M. (1998). Microfabrication of hepatocyte/fibroblast co-cultures: role of homotypic cell interactions. Biotechnology Progress 14(3), 378-387.

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The science and engineering of stem cell-derived organoids-examples from hepatic, biliary, and pancreatic tissues

Affiliations

The science and engineering of stem cell-derived organoids-examples from hepatic, biliary, and pancreatic tissues

Authors

Affiliations

Abstract

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References

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