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. 2020 Aug 28:7:554.
doi: 10.3389/fvets.2020.00554. eCollection 2020.

Spatial Distribution of Trypanosomes in Cattle From Western Kenya

Velma Kivali  1   2 , Alice N Kiyong'a  1 , Jenna Fyfe  2 , Philip Toye  1 , Eric M Fèvre  1   3 , Elizabeth A J Cook  1   3
Affiliations

Spatial Distribution of Trypanosomes in Cattle From Western Kenya

Velma Kivali et al. Front Vet Sci. .

Abstract

African Animal Trypanosomiasis (AAT) is a tsetse-transmitted protozoan disease endemic in "the tsetse belt" of Africa. Past studies investigating the epidemiology of the disease rarely focused on spatial distribution when reporting the prevalence. The challenge of understanding the spatial epidemiology of the disease is further confounded by low-sensitive parasitological techniques used in field investigations. This study aimed to identify trypanosome species in cattle and their spatial distribution in western Kenya. Low-sensitive microscopic analysis and highly-sensitive polymerase chain reaction (PCR) techniques were also compared to better understand the epidemiology of Trypanosoma infections by use of the geographical information system (GIS). Blood samples from 888 cattle, collected between August 2010 and July 2012, were examined for Trypanosoma parasites by light microscopy and PCR. The spatial distribution of Trypanosoma positive cases by species were mapped and overlaid on the map for tsetse distribution. The estimated prevalence was 4.17% by PCR compared to 2.48% by microscopy. Trypanosomes were detected in tsetse free areas. Trypanosoma vivax and Trypanosoma b. brucei were identified, but not the zoonotic Trypanosoma b. rhodesiense. This study demonstrated the importance of geospatial data analysis to understand the epidemiology of the parasite, to inform future research and formulate control strategies.

Keywords: Kenya; cattle; spatial distribution; trypanosomiasis; tsetse.

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Figures

Figure 1
Figure 1
Map of the distribution of homesteads with trypanosome positive cattle in Busia region detected by (A) Microscopy, (B) PCR, and (C) Microscopy and PCR.
Figure 2
Figure 2
Spatially-smoothed risk map for trypanosomes in cattle in Busia region detected by (A) Microscopy, (B) PCR, and (C) Microscopy and PCR. The points indicate locations of hometeads with positive trypanasome samples. Risk is measured as a ratio between 0 and 1 with results closest to 1 having the highest risk and demonstrated by increasing color intensity.
See this image and copyright information in PMC

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