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. 2020 Dec 15:206:111392.
doi: 10.1016/j.ecoenv.2020.111392. Epub 2020 Sep 29.

Pre- sowing seed treatment with salicylic acid and sodium hydrosulfide confers Pb toxicity tolerance in maize (Zea mays L.)

Roya Zanganeh  1 , Rashid Jamei  1 , Fatemeh Rahmani  2
Affiliations

Pre- sowing seed treatment with salicylic acid and sodium hydrosulfide confers Pb toxicity tolerance in maize (Zea mays L.)

Roya Zanganeh et al. Ecotoxicol Environ Saf. .

Abstract

In the present study, impact of salicylic acid (SA), sodium hydrosulfide (NaHS) and concomitant application of SA and NaHs seed priming was investigated in alleviation of the lead stress adverse effects on growth parameters, total chlorophyll content, dicarbonyl stress, and lead-induced iron deficiency in maize. Maize seeds were soaked in 0.5 mM SA and 0.5 mM NaHS individually and in 0.25 mM SA and 0.25 mM NaHS concomitantly for 12 h. The 6 day old plants were subjected to 2.5 mM Pb(NO3)2 for 9 days. Lead stress caused a significant decrease in growth parameters and total chlorophyll and shoot iron contents, whereas increased lead and methylglyoxal accumulation significantly. The cysteine and methionine contents elevated in shoots of lead stressed plants; probably due to redirection of sulfur assimilation requirement for lead detoxification. The SA, NaHS and SA+NaHS applications modulated glyoxalase I activity and resulted in remarkable reduction in methylglyoxal accumulation during lead stress. Lead induced iron deficiency was reverted under SA, NaHS and SA+NaHS seed priming in shoots, probably through reduction in lead uptake and increase in nitric oxide content. Lead imposition activated iron starvation pathway via elevation in methionine content and expression of iron uptake and hemostasis-related genes including Yellow Stripe1 (ZmYS 1), S-adenosylmethionine synthase (ZmSAMS) and 2'-deoxymugineic acid synthase (ZmDMAS1) in roots. However, the ZmSAMS and ZmDMAS1 transcript levels did not change under lead exposure in shoots. The SA, NaHS and SA+NaHS seed primed plants displayed downregulation of ZmSAMS and ZmDMAS1 in shoots and roots under lead stress. In conclusion, seed priming with SA and NaHS could improve lead tolerance in maize via reduction in the Pb uptake, consequently lowering lead toxicity in the food chain.

Keywords: Fe deficiency; Glyoxalase system, Hydrogen sulfide; Lead stress; Salicylic acid; Zea mays.

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