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Review
. 2020;28(6):2547-2559.
doi: 10.1007/s10499-020-00610-4. Epub 2020 Sep 26.

Diagnostic approaches and contribution of next-generation sequencing technologies in genomic investigation of Vibrio parahaemolyticus that caused acute hepatopancreatic necrosis disease (AHPND)

Lean Huat Yu  1 , Cindy Shuan Ju Teh  2 , Kien Pong Yap  1 , Kwai Lin Thong  3
Affiliations
Review

Diagnostic approaches and contribution of next-generation sequencing technologies in genomic investigation of Vibrio parahaemolyticus that caused acute hepatopancreatic necrosis disease (AHPND)

Lean Huat Yu et al. Aquac Int. 2020.

Abstract

A unique strain of Vibrio parahaemolyticus (designated as VPAHPND) causes acute hepatopancreatic necrosis disease (AHPND), a deadly bacterial disease associated with mass mortality in cultured shrimps since 2009. AHPND is responsible for severe economic losses worldwide, causing multimillion-dollar loss annually. Because of the rapid and high mortality rates in shrimps, substantial research has been carried out to develop rapid detection techniques. Also, recent technological advances such as the next-generation sequencing (NGS) have made it possible to elucidate relevant information about a pathogen in a single assay. This review summarizes the current research pertaining to VPAHPND, focusing on diagnosis and contribution of NGS technologies in the genomic studies of AHPND.

Keywords: Acute hepatopancreatic necrosis disease; Diagnostics; Next-generation sequencing; Vibrio parahaemolyticus.

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Conflict of interest statement

Conflict of interestThe authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Emergence of AHPND in different regions around the world. AHPND outbreaks have been reported in shrimp farms in China (2009), Vietnam (2010), Malaysia (2011), Thailand (2012), Mexico (2014), Philippines (2015) and Latin America (2016)
See this image and copyright information in PMC

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