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. 2020 Sep 18;5(38):24239-24246.
doi: 10.1021/acsomega.0c02040. eCollection 2020 Sep 29.

Acceleration of Wound Healing in Diabetic Rats through Poly Dimethylaminoethyl Acrylate-Hyaluronic Acid Polymeric Hydrogel Impregnated with a Didymocarpus pedicellatus Plant Extract

Amit K Mittal  1   2 , Rohit Bhardwaj  1 , Riya Arora  1 , Aarti Singh  3 , Monalisa Mukherjee  3 , Satyendra K Rajput  1   2
Affiliations

Acceleration of Wound Healing in Diabetic Rats through Poly Dimethylaminoethyl Acrylate-Hyaluronic Acid Polymeric Hydrogel Impregnated with a Didymocarpus pedicellatus Plant Extract

Amit K Mittal et al. ACS Omega. .

Abstract

Wound is the major health problem associated with skin damages and arises because of various types of topical injuries. Furthermore, wounds in patients with diabetes take a relatively long time to heal. Currently, herbal medicines have been extensively used for wound care and management. Here, we engineered polymeric hybrid hydrogel of dimethylaminoethyl acrylate and hyaluronic acid (pDMAEMA-HA), which was impregnated with a herbal extract of Didymocarpus pedicellatus. The developed polymeric hybrid hydrogel system can be used for effective therapy of incurable wounds. Therefore, the development of D. pedicellatus-impregnated pDMAEMA-HA (pDPi-DMAEMA-HA) hybrid hydrogel was accomplished by the synthesis of pDMAEMA-HA hydrogel via the optimization of various reaction parameters followed by impregnation of herbal drugs D. pedicellatus. The developed hydrogel composite was well characterized via various techniques, and swelling kinetics was performed to analyze the water uptake property. The swelling ratio was found to be 1600% in both types of hydrogels. To evaluate the wound healing of these polymeric hydrogels, the Wistar rats full-thickness excision wound model was utilized. The healing strength of hydrogels was determined using measurement of wound contraction and histopathological study. The results of wound healing by these polymeric hydrogels revealed that animals treated with the pDPi-DMAEMA-HA hybrid hydrogel group were found to have a higher level of wound closure as compared to marketed formulation as well as polymeric hybrid hydrogel. The histopathologic examinations implied that pDPi-DMAEMA-HA hybrid hydrogel and polymeric hybrid hydrogel-treated groups exhibited enhanced cutaneous wound repair as well as high level of cellular repair and maintenance compared to the standard group because of hyaluronic acid roles in various stages of wound repair.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Chemical structure of (a) dimethylaminoethyl acrylate (DMAEMA) and (b) hylauronic Acid (HA).
Figure 2
Figure 2
SEM micrographs of (a) Cross section of pDMAEMA–HA hydrogels and (b) cross section of pDPi-DMAEMA–HA hydrogels.
Figure 3
Figure 3
FTIR spectroscopy micrographs overlay of the (a) D. pedicellatus plant extract, (b) pDMAEMA–HA hydrogels, and (c) pDPi-DMAEMA–HA hydrogels.
Figure 4
Figure 4
Swelling percentage with time (h) in buffer solution of (a) pDMAEMA–HA hydrogels and (b) pDPi-DMAEMA–HA hydrogels.
Figure 5
Figure 5
Visual inspection of wound healing in rats (a) disease group, (b) treated with standard marketed formulation, (c) treated with pDMAEMA–HA hydrogels (treatment 1), and (d) treated with pDPi-DMAEMA–HA hydrogels (treatment 2).
Figure 6
Figure 6
Percent of wound closure after days (a) disease group, (b) treated with standard marketed formulation, (c) treated with pDMAEMA–HA hydrogels (treatment 1), and (d) treated with pDPi-DMAEMA–HA hydrogels (treatment 2).
Figure 7
Figure 7
Histopathology of the rat skin (a) normal control, (b) disease control, (c) standard treatment, (d) treated with pDMAEMA–HA hydrogels (treatment 1), and (e) treated with pDPi-DMAEMA–HA hydrogels (treatment 2).
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