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. 1977 Jul 15;33(7):972-3.
doi: 10.1007/BF01951312.

Establishment of serum based essentiall free of proteolytic activity for the culture of mouse pancreatic islets

M Kedinger, A Moody, J F Launay, K Haffen

Establishment of serum based essentiall free of proteolytic activity for the culture of mouse pancreatic islets

M Kedinger et al. Experientia. .

Abstract

The present study demonstrates that a) serum based culture medium degrades 125I inhibits its proteolytic activity leading to the recovery of more insulin secreted by islets cultured in the presence of high glucose concentration alone or with glucagon; c) aprotinin also favoured the accumulation of secreted insulin by protecting the hormone from a residual degradative capacity of the hear treated serum.

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References

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