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. 2020 Nov;17(11):1093-1096.
doi: 10.1038/s41592-020-0966-x. Epub 2020 Oct 5.

An efficient KRAB domain for CRISPRi applications in human cells

Nader Alerasool  1   2 , Dmitri Segal  1   2 , Hunsang Lee  1 , Mikko Taipale  3   4   5
Affiliations

An efficient KRAB domain for CRISPRi applications in human cells

Nader Alerasool et al. Nat Methods. 2020 Nov.

Abstract

Clustered regularly interspaced short palindromic repeat interference (CRISPRi), based on the fusion of inactive Cas9 (dCas9) to the Krüppel-associated box (KRAB) repressor, is a powerful platform for silencing gene expression. However, it suffers from incomplete silencing of target genes. We assayed 57 KRAB domains for their repressive potency and identified the ZIM3 KRAB domain as an exceptionally potent repressor. We establish that ZIM3 KRAB-dCas9 fusion silences gene expression more efficiently than existing platforms.

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References

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