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Comment
. 2020 Oct 6;5(1):222.
doi: 10.1038/s41392-020-00317-1.

Is smaller better? Vaccine targeting recombinant receptor-binding domain might hold the key for mass production of effective prophylactics to fight the COVID-19 pandemic

Manish Muhuri  1   2 , Guangping Gao  3   4   5
Affiliations
Comment

Is smaller better? Vaccine targeting recombinant receptor-binding domain might hold the key for mass production of effective prophylactics to fight the COVID-19 pandemic

Manish Muhuri et al. Signal Transduct Target Ther. .
No abstract available

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Conflict of interest statement

G.G. is a scientific co-founder of Aspa Therapeutics and Voyager Therapeutics and holds equity in the companies. G.G. is an inventor of patents related to adeno-associated virus (AAV) gene therapy with potential royalties licensed to Aspa Therapeutics, Voyager Therapeutics and other biopharmaceutical companies.

Figures

Fig. 1
Fig. 1
Design and production of an RBD-based vaccine and inspecting its protective effects in in vivo models. a The Spike protein of SARS-CoV-2 is known to be critical for virus’ entry into the cells. Rational design of the vaccine involves the insertion of a GP67 signal peptide upstream to the RBD domain of the spike protein. The recombinant protein is produced using insect cells and the Bac-to-Bac baculovirus expression system. The purified protein formulation is then injected via intramuscular injections in mice, rabbits, and non-human primates (NHPs; Macaca mulatta). The sera from the injected animals are immunophenotyped to profile the immune response against the RBD protein. IgG, IgM, and memory lymphocytes are consistently produced across all immunized animals. The indicated domains and elements of the spike protein, including signal sequence (SS), N-terminal domain (NTD), receptor-binding domain (RBD), heptad repeat1 and 2 (HR1 and HR2), transmembrane domain (TM), and cytoplasmic domain (CT), are marked. b NHPs are sequentially immunized on day 0 and day 7 intramuscularly with purified RBD or control treatment followed by intranasal challenge with live SARS-CoV-2 on day 28. Neutralization activity was measured from their sera, viral load was measured from throat, lung, and anal swabs, and histopathological changes in lung tissue were analyzed by light microscopy. Immunized NHPs displayed potent neutralization, minimal viral load with the appearance of normal lung tissue histopathology
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Comment on

  • A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity.
    Yang J, Wang W, Chen Z, Lu S, Yang F, Bi Z, Bao L, Mo F, Li X, Huang Y, Hong W, Yang Y, Zhao Y, Ye F, Lin S, Deng W, Chen H, Lei H, Zhang Z, Luo M, Gao H, Zheng Y, Gong Y, Jiang X, Xu Y, Lv Q, Li D, Wang M, Li F, Wang S, Wang G, Yu P, Qu Y, Yang L, Deng H, Tong A, Li J, Wang Z, Yang J, Shen G, Zhao Z, Li Y, Luo J, Liu H, Yu W, Yang M, Xu J, Wang J, Li H, Wang H, Kuang D, Lin P, Hu Z, Guo W, Cheng W, He Y, Song X, Chen C, Xue Z, Yao S, Chen L, Ma X, Chen S, Gou M, Huang W, Wang Y, Fan C, Tian Z, Shi M, Wang FS, Dai L, Wu M, Li G, Wang G, Peng Y, Qian Z, Huang C, Lau JY, Yang Z, Wei Y, Cen X, Peng X, Qin C, Zhang K, Lu G, Wei X. Yang J, et al. Nature. 2020 Oct;586(7830):572-577. doi: 10.1038/s41586-020-2599-8. Epub 2020 Jul 29. Nature. 2020. PMID: 32726802

References

    1. Yang, J. et al. A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity. Nature10.1038/s41586-020-2599-8 (2020). - PubMed
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    1. Premkumar, L. et al. The receptor binding domain of the viral spike protein is an immunodominant and highly specific target of antibodies in SARS-CoV-2 patients. Sci. Immunol. 10.1126/sciimmunol.abc8413 (2020). - PMC - PubMed

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