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. 2021 Nov 9;45(9):950-956.
doi: 10.1093/jat/bkaa147.

Blood Phosphatidylethanol Concentrations Following Regular Exposure to an Alcohol-Based Mouthwash

Gary M Reisfield  1 , Scott A Teitelbaum  2 , Joseph T Jones  3 , Dana Mason  1 , Max Bleiweis  1 , Ben Lewis  2
Affiliations

Blood Phosphatidylethanol Concentrations Following Regular Exposure to an Alcohol-Based Mouthwash

Gary M Reisfield et al. J Anal Toxicol. .

Abstract

Direct biomarkers of ethanol are used to monitor individuals who are required to abstain from ethanol consumption. In recent years, blood phosphatidylethanol (PEth) has gained acceptance in clinical and forensic contexts as an abstinence marker. Its elimination half-life of several days provides a window of detection of days to weeks. However, there is no research addressing the extent of PEth formation related to extraneous ethanol exposures. To assess the degree of ethanol absorption and subsequent formation of blood PEth related a common extraneous exposure, regular use of an ethanol-containing mouthwash, we recruited 16 participants to gargle with an alcohol-based mouthwash (21.6% ethanol) 4 times daily, for 12 consecutive days. Blood was analyzed for PEth 16:0/18:1 by liquid chromatography-tandem mass spectrometry. Our hypothesis that blood PEth concentrations would not equal or exceed 20 ng/mL was confirmed. Although the data suggest that regular use of mouthwash is unlikely to result in suprathreshold PEth concentrations, this work highlights the importance of considering extraneous ethanol exposures in clinical decision-making and in future research.

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Figures

Figure 1.
Figure 1.
List of products participants were instructed to avoid.
Figure 2.
Figure 2.
Specimen collection timeline.
See this image and copyright information in PMC

References

    1. Gustavsson L. (1995) ESBRA 1994 award lecture. Phosphatidylethanol formation: specific effects of ethanol mediated via phospholipase D. Alcohol and Alcoholism, 30, 391–406. - PubMed
    1. Isaksson A., Walther L., Hansson T., Anderson A., Alling C. (2011) Phosphatidylethanol in blood (B-PEth): a marker for alcohol use and abuse. Drug Testing and Analysis, 3, 195–200. doi: 10.1002/dta.278 - DOI - PubMed
    1. Helander A., Bottcher A., Dahman N., Beck O. (2019) Elimination characteristics of the alcohol biomarker phosphatidylethanol (PEth) in blood during alcohol detoxification. Alcohol and Alcoholism, 54, 251–257. doi: 10.1093/alcalc/agz027 - DOI - PMC - PubMed
    1. Javors M.A.,., Hill-Kapturcazk N., Roache J.D., Karns-Wright T.E., Dougherty D.M. (2016) Characterization of the pharmacokinetics of phosphatidylethanol 16:0/18:1 and 17:0/18:2 in human whole blood after alcohol consumption in a clinical laboratory study. Alcoholism, Clinical and Experimental Research, 40, 1228–1234. - PMC - PubMed
    1. Mueller G.C., Fleming M.F., LeMahieu M.A., Lybrand G.S., Barry K.J. (1988) Synthesis of phosphatidylethanol—a potential marker for adult males at risk for alcoholism. Proceedings of the National Academy of Sciences, 85, 9778–9782. doi: 10.1073/pnas.85.24.9778 - DOI - PMC - PubMed